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Plasmid#67180PurposeCRISPaint universal donor for gene expression quantification using destabilized TurboGFPDepositorInsertT2A-TurboGFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-NAT-LACap
Plasmid#80763PurposeExpresses truncated L-A capsid for curing satellite virusDepositorInsertL-A cDNA (L-A Cap)
UseTagsExpressionYeastMutationPromoterPGK1Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTEF1-yEGFPCLN2PEST-pRS406
Plasmid#64406PurposeTEF promoter driven GFP expressionDepositorInsertTEF1 promoter (TEF1 Budding Yeast)
UseTagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaD (pESC106)
Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaE (pESC124)
Plasmid#50390PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertDPCK
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSAD-F3-NPEST-mCherry
Plasmid#104332PurposeSelf-inactivating DG-Rabies (SiR) expressing mCherryDepositorInsertmCherry
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1-Hypa
Plasmid#218166PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mVenus-RapR-PTPPest
Plasmid#188657PurposeRapamycin regulated PTPPest with mVenus tagDepositorInsertProtein Tyrosine Phosphatase Pest (PTPN12 )
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
UseTagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-PmCDA1
Plasmid#60818Purposeto overexpress lamprey deaminase in yeastDepositorInsertCDA1
UseTagsFLAGExpressionYeastMutationPromoterGAL10Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorInsertAID (AICDA Human)
UseTagsMycExpressionYeastMutationcodon optimized for yeastPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
5787 pET28aSX104B/ pES42
Plasmid#1229DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsHisExpressionBacterialMutationnonePromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-SopB
Plasmid#183670PurposeInducible Salmonella Typhimurium SopB for expression in yeastDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PSUC2-yEGFPCLN2PEST-pRS406
Plasmid#64405PurposeSUC2 promoter driven GFP expressionDepositorInsertSUC2 promoter (SUC2 Budding Yeast)
UseTagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastMutationPromoterSUC2Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Clux
Plasmid#47658PurposePesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)DepositorInsertPesaR-C
UseSynthetic BiologyTagsExpressionBacterialMutationAdditional esa box between -35 site and the origi…PromoterPesaR-CAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pES002 (pTet-qacR wt)
Plasmid#69031Purposewild-type qacR downstream of a Tet promoterDepositorInsertqacR (qacR Synthetic)
UseTagsHis6 tagExpressionBacterialMutationPromoterpTetAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Plux
Plasmid#47659PurposePesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-P
UseSynthetic BiologyTagsExpressionBacterialMutationAdditional esa box downstream of the original esa…PromoterPesaR-PAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS-PesaR-Plux
Plasmid#47655PurposePesaR-P promoter in pCS26DepositorInsertPesaR-P
UseSynthetic BiologyTagsExpressionBacterialMutationAdded an additional esa box downstream of the ori…PromoterPesaR-PAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU2-RAD30cmyc
Plasmid#60821Purposeto overexpress yeast polymerase eta in yeastDepositorInsertRAD30 (RAD30 Budding Yeast)
UseTagsc-mycExpressionYeastMutationPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Dlux
Plasmid#47657PurposePesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-D
UseSynthetic BiologyTagsExpressionBacterialMutationAdded an additional esa box upstream of -35 site …PromoterPesaR-DAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only