We narrowed to 1,196 results for: post

1. pH6HTC_RAN-Halo

   Plasmid

   #175339

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   RAN (RAN Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-GG-PUF

   Plasmid

   #52137

   Purpose

   Receiving vector for PUM-HD assembly and expression in E.coli

   Depositor

   Huimin Zhao

   Article

   Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

   Type

   Empty backbone

   Use

   Tags

   PUM-HD flanking region 1' and PUM-HD flankin…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTRE2_CMV_Mex3a_t2a_mCherry

   Plasmid

   #223244

   Purpose

   tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry

   Depositor

   Stavros Lomvardas

   Insert

   Mex3a (Mex3a Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   amino acids conserved, underlying DNA sequence re…

   Promoter

   CMV

   Available since

   May 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

   Plasmid

   #225438

   Purpose

   Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

   Depositor

   Diego Orzaez

   Insert

   CBS-FRT-OCS-BeYDVRepRepA-tNos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

   Plasmid

   #225436

   Purpose

   BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

   Depositor

   Diego Orzaez

   Insert

   BeYDV geminino 1.0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

   Plasmid

   #225430

   Purpose

   OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

   Depositor

   Diego Orzaez

   Insert

   miniDFR-FRT-OCSterm-FRT-miniDFR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

   Plasmid

   #225431

   Purpose

   OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter

   Depositor

   Diego Orzaez

   Insert

   miniDFR-att-OCSterm-att-miniDFR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)

   Plasmid

   #225439

   Purpose

   Module for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

   Depositor

   Diego Orzaez

   Insert

   HygR-MAR10-gemiGFP-MAR10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH6HTC_mdHUR-Halo

   Plasmid

   #175312

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HUR
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAKW.031-GM-R5

    Plasmid

    #154044

    Purpose

    Expresses R5 peptide in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Silaffin R5 peptide
    

    Use

    Tags

    Maltose binding protein and his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pH6HTC_TKT-Halo

    Plasmid

    #175331

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    TKT (TKT Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 V5 hHR23B

    Plasmid

    #13054

    Depositor

    Steven Grossman

    Article

    Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

    Insert

    hHR23B (RAD23B Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. nSYB-GAL80-DD

    Plasmid

    #124778

    Purpose

    Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GAL80 (GAL80-DD)
    

    Use

    Tags

    DD

    Expression

    Insect

    Mutation

    Promoter

    nSynaptobrevin

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pH6HTC_PTBP1-Halo

    Plasmid

    #175302

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    PTBP1 (PTBP1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NODAL-matMYC

    Plasmid

    #115248

    Purpose

    For mammalian expression of the human NODAL open reading frame with an internal MYC tag

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 599 p3E-mcs1

    Plasmid

    #49004

    Purpose

    Multiple cloning site in 3' gateway entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Gateway 3' entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 10xUAS-GFP-DD

    Plasmid

    #104989

    Purpose

    Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GFP (GFP-DD)
    

    Use

    Tags

    DD

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAVS1-TRE3G-NODAL

    Plasmid

    #115638

    Purpose

    For targeted integration and inducible expression of human NODAL using doxycycline

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXCX.CMV.tTA

    Plasmid

    #38056

    Depositor

    James Uney

    Article

    Lee et al J Gene Med. 2005 May;7(5):576-83.

    Insert

    tTA
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-C3orf17-gfp

    Plasmid

    #70265

    Purpose

    Expresses C3orf17 in mammalian cells with GFP reporter.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    C3orf17 (NEPRO Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only