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We narrowed to 923 results for: tac promoter

Showing: 61 - 80 of 923 results

  2. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Po (phoenol-inducible promoter)
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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  4. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188745
    Purpose
    Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nonTATAchr21
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188745-plasmid-map-sequence-id-371435

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  5. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188746
    Purpose
    Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nonTATAchrX
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188746-plasmid-map-sequence-id-373413

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  14. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter from pKK233-2
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62343-plasmid-map-sequence-id-102266

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  15. pSEVA47_dCas9-GFP_NOT_iC

    Plasmid
    #231418
    Purpose
    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)
    Depositor
    Alfonso Jaramillo
    Article
    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev
    Inserts
    dCas9
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    231418-plasmid-map-sequence-id-468903

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  17. ST-2xTstem(5A)-GFP

    Plasmid
    #162504
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162504-plasmid-map-sequence-id-323646

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  18. ST-2xTstem-GFP

    Plasmid
    #162503
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162503-plasmid-map-sequence-id-323511

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  19. NSSCP

    Plasmid
    #183190
    Purpose
    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV
    Depositor
    Hsiao-Chun Huang
    Article
    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1
    Inserts
    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183190-plasmid-map-sequence-id-366594

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  20. ST-Tstem(5A)-GFP

    Plasmid
    #162502
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The mutated Tac stem region is inserted within ST…
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162502-plasmid-map-sequence-id-323507

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Showing: 61 - 80 of 923 results