We narrowed to 5,091 results for: codon optimized

1. pDONR221-LETMD1_STOP

   Plasmid

   #161460

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   LETMD1 (LETMD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDONR221-MAGT1_STOP

   Plasmid

   #161468

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MAGT1 (MAGT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJDC181

   Plasmid

   #60853

   Purpose

   Expresses click beetle red luciferase

   Depositor

   Jeffrey Cirillo

   Article

   Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

   Insert

   Click beetle red luciferase (optimized for Mycobacteria)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for Mycobacteria

   Promoter

   hsp60

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJDC182

   Plasmid

   #60854

   Purpose

   Expresses firefly luciferase

   Depositor

   Jeffrey Cirillo

   Article

   Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

   Insert

   Firefly luciferase (optimized for Mycobacteria)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for Mycobacteria

   Promoter

   hsp60

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. DinoIII-arrO-N

   Plasmid

   #128143

   Purpose

   Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

   Depositor

   Senjie Lin

   Article

   Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

   Insert

   Rifampin ADP-ribosylating transferase + potential promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-CAG-dCas9-VPR-wpA-pPuroTK

   Plasmid

   #214127

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUbi_dCas9-BFP-KRAB-gRNA

   Plasmid

   #221503

   Purpose

   CRISPRi machinery fish codon optimized expressing plasmid

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Zebrafish Ubiquitin promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHN872

   Plasmid

   #178830

   Purpose

   Bacterial expression for human Saposin A

   Depositor

   Kazuhiro Abe

   Insert

   artificial gene encoding human Saposin A
   

   Use

   Tags

   GST, His8, TEV

   Expression

   Bacterial

   Mutation

   Codon-usage was optimized for bacterial expressio…

   Promoter

   T7

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-ABE7.10-P2A-Puro

   Plasmid

   #112674

   Purpose

   Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

   Depositor

   Lukas Dow

   Insert

   ABE7.10
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b-ACP (from S. aureus)

    Plasmid

    #63686

    Purpose

    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus

    Depositor

    Michael Johnson

    Insert

    ACP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pFA6a-pTagRFP-T-kanMX6

    Plasmid

    #240555

    Purpose

    Tag S. pombe genes at 3' end (C-terminal end of protein) with TagRFP-T, codon-optimized for S. pombe

    Depositor

    Silke Hauf

    Insert

    pTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMG005_mNeonGreen_P2A

    Plasmid

    #237215

    Purpose

    Fluorescent reporter codon optimized for Dictyostelid species

    Depositor

    Jordi van Gestel

    Insert

    mNeonGreen-P2A
    

    Use

    Cloning

    Tags

    3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMG008_mCherry_P2A

    Plasmid

    #237216

    Purpose

    Fluorescent reporter codon optimized for Dictyostelid species

    Depositor

    Jordi van Gestel

    Insert

    mCherry-P2A
    

    Use

    Cloning

    Tags

    3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

    Plasmid

    #234950

    Purpose

    Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid

    #234951

    Purpose

    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid

    #141347

    Purpose

    3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

    Depositor

    Gerald Pao

    Article

    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

    Insert

    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    resynthesized with human codon optimization nucle…

    Promoter

    CAG

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNMSB104

    Plasmid

    #199314

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LeGO-iBSD-5LO-WT

    Plasmid

    #182741

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSH-EFIRES-P-GFP(1-10)opti

    Plasmid

    #129416

    Purpose

    Expressing codon-optimized GFP(1-10) fragment in human cells

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    codon-optimized GFP(1-10)
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid

    #157732

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nprot-HF_GC3opt (N SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid

    #157702

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

    Plasmid

    #157701

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid

    #114090

    Purpose

    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized MbCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTE3328

    Plasmid

    #107539

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K607R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge