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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 156,026 results for: ins;

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Showing: 8001 - 8020 of 156026 results

  1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid
    #208954
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208954-plasmid-map-sequence-id-415743

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  15. G protein alpha-s-YFP

    Plasmid
    #55781
    Purpose
    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-s-EE YFP (Gnas Rat)
    Tags
    EYFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    Met was substituted for Gln69 in EYFP (Clontech).…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55781-plasmid-map-sequence-id-119279

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  20. pMal_Abl-PRM 3R

    Plasmid
    #112086
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-3R (ABL1 Human)
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112086-plasmid-map-sequence-id-236089

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Showing: 8001 - 8020 of 156026 results