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pSP64-hERG1a

Plasmid

#53051

Purpose

Expresses alpha subunit of hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pTA7002-avrPto

Plasmid

#49156

Purpose

Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto

Depositor

Sheng Yang He

Article

Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Insert

avrPto

Use

Plant transformation binary vector

Promoter

dexamethasone inducible

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pBAD/HisB-Fast-FT

Plasmid

#31916

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Tags

His

Expression

Bacterial

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

araB

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB Q72L

Plasmid

#19721

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Mutation

Q72L constitutively active, GTPase Deficient

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO DNAJB1

Plasmid

#19468

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB1 (DNAJB1 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pMIK-Neo-mYes

Plasmid

#18067

Depositor

Marius Sudol

Article

Espanel et al J Biol Chem. 2001 Apr 27. 276(17):14514-

Insert

yes kinase (Yes1 Mouse)

Expression

Mammalian

Available Since

July 10, 2008

Availability

Academic Institutions and Nonprofits only
pLVX-IRES-Hygro_GCaMP6s

Plasmid

#164591

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαq-LgB97

Plasmid

#134360

Purpose

Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαq-LgB97 (GNAQ Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)

Plasmid

#145147

Purpose

Mammalian expression plasmid for soluble ACE2 (protease domain)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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Rab8WT(Flag) in pcDNA3.1neo

Plasmid

#46783

Purpose

expression of WT Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A (RAB8A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-ATBF1

Plasmid

#40927

Depositor

Jin-Tang Dong

Article

Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

Insert

ATBF1 (ZFHX3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-101-FLAG

Plasmid

#35981

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 101 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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T7-p73DD-pcDNA3

Plasmid

#22978

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p73 (TP73 Human)

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 327-…

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA1

Plasmid

#22507

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA1 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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PKC alpha dNPS

Plasmid

#21233

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Mutation

delta 1-29

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
TRX1 flag C35S

Plasmid

#21285

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

Flag

Expression

Mammalian

Mutation

C35S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pMKO.1 GFP B56 gamma3 shRNA 14-1

Plasmid

#10681

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma3 shRNA (PPP2R5C Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pOpen-phi29pol

Plasmid

#165573

Purpose

DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

phi29 DNA Polymerase

Use

Synthetic Biology

Available Since

Aug. 24, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.GFAP.iGABASnFR

Plasmid

#112172

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

AAV

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKW08-Lx-Int

Plasmid

#25015

Depositor

Brian Robertson

Article

Williams et al Plasmid. 2010 Sep;64(2):69-73. doi: 10.1

Insert

pKW08-Lx-Int

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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