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Ai32

Plasmid

#34880

Purpose

Cre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitation

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

Floxed ChR2(H134R)-EYFP

Use

Mouse Targeting

Tags

EYFP

Mutation

H134R in ChR2

Promoter

CAG

Available Since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD9-pHluorin

Plasmid

#130905

Purpose

Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD9-pHluorin (CD9 Human, Aequorea victoria)

Tags

pHluorin

Expression

Mammalian

Mutation

phluorin inserted between Asn-50 and Asn-51 in ex…

Promoter

CMV

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR H215R AAA mutant

Plasmid

#13510

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR H215R AAA (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only
pRS40H

Plasmid

#35479

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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OCT4-2A-mOrange

Plasmid

#66986

Purpose

Generate OCT4 mOrange reporter line without selection cassette

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

2A-mOrange

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-PRAS40

Plasmid

#15481

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3_MERS_D12

Plasmid

#170448

Purpose

Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

MERS spike D12 (S MERS)

Expression

Mammalian

Mutation

last 12aa deletion in c-terminal tail

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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M3-M14-dCas9

Plasmid

#134779

Purpose

Expression of fusion protein M3-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 Human, S. pyogenes)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet1 CD

Plasmid

#70129

Purpose

Express flag-tagged mouse Tet1 catalytic domain.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain.

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-L26-Flag

Plasmid

#19972

Depositor

Moshe Oren

Article

Ofir-Rosenfeld et al Mol Cell. 2008 Oct 24. 32(2):180-9.

Insert

Rpl26 (RPL26 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only
pCB7 alpha5

Plasmid

#16041

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

alpha5 (ITGA5 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pAG303-Gal-GR100

Plasmid

#84908

Purpose

induce expression of dipeptide repeat GR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GR100 (C9orf72 Synthetic)

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-PCAF

Plasmid

#63705

Purpose

retroviral expression of human PCAF in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

PCAF (KAT2B Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

E452G

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFS461

Plasmid

#89064

Purpose

leu1 integration plasmid with adh1pr:Z3EV

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV artificial transcription factor

Use

Leu1 integrating vector

Promoter

adh1

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTn Donor

Plasmid

#236211

Purpose

Arabinose-inducible Tn5 InducTn-seq plasmid

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

None

Expression

Bacterial

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-C

Plasmid

#118369

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pQP-iRIS

Plasmid

#102584

Purpose

Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Backbone(HD)

Plasmid

#27964

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(HD)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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