166,542 results

1. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET23b-15F11-HA-mEGFP-6xHis

   Plasmid

   #129593

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV40-EF1a-hBCL10-SO

   Plasmid

   #75256

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-mitoTPNOX

   Plasmid

   #87854

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   mitoTPNOX
   

   Use

   Tags

   C-terminal FLAG tag with a linker and mitochondri…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AbVec2.0-IGHG1

   Plasmid

   #80795

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2.5-THP-GFP

   Plasmid

   #80336

   Purpose

   Fluorescent Reporter for Dopaminergic Neuron Differentiation

   Depositor

   Kwang-Soo Kim

   Article

   Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

   Insert

   rat TH promoter (Th Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   rat Tyrosine hydroxylase

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tandem p40p35

   Plasmid

   #108665

   Purpose

   Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

   Depositor

   Nevil Singh

   Insert

   interleukin-12 (Il12a, Il12b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   IL-12 dimers have a Gly-Ser linker

   Promoter

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAW63.YY1.FKBP.knock-in.BFP

   Plasmid

   #104371

   Purpose

   For knocking in FKBP F36V P2A BFP

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   FKBP12(F36V) -P2A-BFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MaLionB

   Plasmid

   #113910

   Purpose

   Blue ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   MaLionB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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