We narrowed to 10,082 results for: crispr plasmids
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Plasmid#164268PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRExpressionWormPromoterU6 promoter from W05B2.8Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX300A-G+Nanog
Plasmid#140280PurposeCRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locusDepositorInsertCas9
ExpressionMammalianPromoterCBHAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGuide-P1-O1a (EL702)
Plasmid#140040PurposeCRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.DepositorInsertbarcode P1 and sgRNA lacO1 array
UseSynthetic BiologyAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM108
Plasmid#134160PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationKMLL, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM107
Plasmid#134159PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS
Plasmid#107300PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS
Plasmid#107299PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AX44_pS.Donor.S1
Plasmid#100289PurposeAAVS1-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingExpressionMammalianAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-1BPNLS-Cas9-1BPNLS-2AGFP
Plasmid#87109PurposeBPNLS-Cas9-BPNLS-2AGFP expression plasmidDepositorInsert1BPNLS-Cas9-1BPNLS-2A-EGFP
UseCRISPRTags3xFLAG (on Cas9)ExpressionMammalianPromoterCAGAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
ExpressionMammalianAvailable SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDNR-gRNA
Plasmid#206990PurposeA plasmid for expression of SaCas9-sgRNA targeting donor plasmidsDepositorInsertDonor plasmid-targeting SaCas9-gRNA
ExpressionMammalianPromoterU6Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-Cas9
Plasmid#170180PurposeIVT template plasmid for making modified Cas9 RNADepositorInsertCas9
UseIvt template plasmidPromoterT7 promoterAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
7a sgRNA for EJ7-GFP and 4-μHOM reporters
Plasmid#113620PurposesgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reportersDepositorInsert7a sgRNA
UseCRISPRExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
epi-BE4max
Plasmid#135975PurposeExpresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-1xStop
Plasmid#164411PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianPromoterEF1-alphaAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-ePEmax3::tEURb7
Plasmid#227692PurposeGolden Gate level 1, position 2, ePEmax3 expression cassette.DepositorInsertePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRExpressionPlantPromoterCaMV 35SAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS
Plasmid#79373PurposeRecruits VPR to a compatible Cas9 protein for transcriptional activationDepositorInsertGCN4-sfGFP-VPR
UseCRISPRExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrex-n-eGFP
Plasmid#62544PurposeExpression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genomeDepositorInserteGFP
Use(1)trypanosoma cruzi expression of egfp (2) genom…TagsNoneMutationNonePromoterRibo-HX1Available SinceApril 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
ExpressionMammalianPromoterCAGAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
QPM-sgR (pTaU3)
Plasmid#140448PurposeFor sgRNA expression in monocotyledons protoplastsDepositorInsertTaU3-sgRNA
UseCRISPRExpressionPlantPromoterTaU3Available SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only