We narrowed to 10,126 results for: Uty

1. pHR-PGK-LexA-WW-CIB1-biLINuS

   Plasmid

   #171757

   Purpose

   Lentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuS

   Depositor

   Yingxiao Wang

   Article

   Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

   Insert

   Synthetic gene containing LexA-WW-CIB1-biLINuS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACT2-μ3A

   Plasmid

   #198174

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3A
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitution in codon 225

   Promoter

   ADH1

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNHEJ-RPG

   Plasmid

   #85931

   Purpose

   Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

   Depositor

   Zhiying Zhang

   Article

   Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

   Insert

   pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
   

   Use

   Dual-reporter surrogate

   Expression

   Mammalian

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM600 EF1α PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2

   Plasmid

   #161570

   Purpose

   Constitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   PC 3xFLAG-FKBP-FGFR4-TEVp
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe puro Myr HA PIK3CB

   Plasmid

   #12527

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele.

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_tKiMBI-T2A-caMEK

   Plasmid

   #199578

   Purpose

   Expresses tKiMBI and caMEK in an AAV vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
   constitutively active MEK
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. nTRb412-gs10-NLS-VPR

   Plasmid

   #188267

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nTRb412 (TXNRD2 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

   Plasmid

   #101351

   Purpose

   HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRE146

   Plasmid

   #118498

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Expression

   Bacterial

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX307 P2A GFP PRDM14

    Plasmid

    #193676

    Purpose

    Constitutive lentiviral expression of PRDM14

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. hCMV-IE1:GLuc:bgH

    Plasmid

    #135294

    Purpose

    Transcriptional unit for constitutive expression of GLuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GLuc:bGH
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUB-sSypHer (RbcS-SypHer)

    Plasmid

    #84732

    Purpose

    Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    sSypHer
    

    Tags

    RbcS targeting sequence

    Expression

    Plant

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHelper-CBE-sgRNA

    Plasmid

    #221137

    Purpose

    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with Cas9 handle
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119(SpeI)

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR3.1 GSK3beta S9A HA

    Plasmid

    #15994

    Depositor

    Morris Birnbaum

    Article

    Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

    Insert

    GSK3beta (GSK3B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A: constitutively active

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Available Since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHES941

    Plasmid

    #87942

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Synthetic, Human)
    

    Expression

    Yeast

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe HcRed Ras

    Plasmid

    #10678

    Depositor

    William Hahn

    Article

    Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

    Insert

    Hras V12 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

20. RIPR-bio-His

    Plasmid

    #50824

    Purpose

    Expresses enzymatically monobiotinylated full length RIPR

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    RIPR
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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