We narrowed to 5,091 results for: codon optimized

1. pTE3329

   Plasmid

   #107538

   Purpose

   Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Lb crRNA
   hLbCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K595R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE3327

   Plasmid

   #107535

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RVR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K548V, N552R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUCIDT-attL1-Worm ABeta-attR5

   Plasmid

   #160437

   Purpose

   Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
   

   Use

   Tags

   Cleavage signal

   Expression

   Bacterial

   Mutation

   Codon-optimized for expression in C. elegans

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE3334

   Plasmid

   #107537

   Purpose

   Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   hMbCpf1(RVR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K582V, N586R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE3330

   Plasmid

   #107534

   Purpose

   Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Lb crRNA
   hLbCpf1(RVR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11d-FHis-DND1

   Plasmid

   #70083

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Use

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE3336

   Plasmid

   #107541

   Purpose

   Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   hMbCpf1(RR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K637R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTE3333

   Plasmid

   #107540

   Purpose

   Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(RR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K671R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTE3331

   Plasmid

   #107536

   Purpose

   Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(RVR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K613V, N617R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTFG002-ACE2(Mut)-HA

    Plasmid

    #191570

    Purpose

    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Angiotensin Converting Enzyme II (ACE2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human expression. Also contai…

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTFG001-ACE2(WT)-HA

    Plasmid

    #191569

    Purpose

    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Angiotensin Converting Enzyme II (ACE2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid

    #192835

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site and 6xhis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. INCTbiosyn-pUB-HspINT7

    Plasmid

    #127515

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 7.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 7 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    In 2126 position an G mutated for an T changed th…

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-Q03JI6

    Plasmid

    #103123

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    

    Use

    CRISPR; Cloning vector

    Tags

    SV40NLS

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. phumanCas9 (GB0575)

    Plasmid

    #68206

    Purpose

    Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIMVAu1

    Plasmid

    #98301

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPQD0CM0539

    Plasmid

    #162306

    Purpose

    Level 0 CDS part for MoClo assembly, AATG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    CDS, TEV protease (S219V), no stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S219V, codon optimized for E. coli

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pet28a_Synthetic_Human_H2A.1

    Plasmid

    #42634

    Depositor

    Joe Landry

    Insert

    Human H2A.1 (H2AC13 Synthetic, Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Codon Optimized

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pPICZaa-STE2-cohis

    Plasmid

    #133433

    Purpose

    Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S/C252A, Codon optimized for P. pastoris, and …

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBHM2642

    Plasmid

    #229526

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ecALDH

    Plasmid

    #67921

    Purpose

    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Insert

    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJJR81

    Plasmid

    #75029

    Purpose

    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Insert

    tagBFP2^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized tagBFP2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-CAG-mNeonGreen

    Plasmid

    #124101

    Purpose

    Expression of the fluorescent protein mNeonGreen in mammalian cells

    Depositor

    Pantelis Tsoulfas

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage was optimized for Homo Sapiens

    Promoter

    CAG

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS-L1PA1CH blast

    Plasmid

    #69612

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Use

    Tags

    blast cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS-L1PA13ACHmneo

    Plasmid

    #69613

    Purpose

    Expresses a neomycin tagged codon optimized L1PA13A

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA13A
    

    Use

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCR182

    Plasmid

    #240642

    Purpose

    Template for in vitro transcription of AmNeonGreen mRNA using SP6 polymerase.

    Depositor

    John Pringle

    Insert

    AmNeonGreen
    

    Use

    Vertebrate gene expression and general in vitro t…

    Tags

    Expression

    Mutation

    Codon-optimized for Exaiptasia diaphana, amino ac…

    Promoter

    SP6

    Available since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBHM2643

    Plasmid

    #229527

    Purpose

    Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mAID_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103 STXBP4 WW domain

    Plasmid

    #104265

    Purpose

    Bacterial expression of WW domain from STXBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    STXBP4 WW domain (STXBP4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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