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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 801 - 820 of 23858 results

  2. pfMDH_K381A

    Plasmid
    #67732
    Purpose
    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 381 to alanine
    Promoter
    T7
    Available Since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pfMDH_N300D

    Plasmid
    #67728
    Purpose
    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to aspartate
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pfMDH_R373A

    Plasmid
    #67731
    Purpose
    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed arginine 373 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pfMDH_N300S

    Plasmid
    #67729
    Purpose
    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to serine
    Promoter
    T7
    Available Since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pCVD437

    Plasmid
    #63758
    Purpose
    7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Expression
    Bacterial
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pCVD434

    Plasmid
    #63757
    Purpose
    EPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae probe
    Expression
    Bacterial
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. Clomeleon

    Plasmid
    #203723
    Purpose
    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration
    Depositor
    George Augustine
    Article
    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1
    Insert
    cyan fluorescent protein linked yellow florescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cyan Fluorescent Protein, Topaz Fluorescent Prote…
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203723-plasmid-map-sequence-id-403632

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Showing: 801 - 820 of 23858 results