We narrowed to 20,158 results for: Nov
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Plasmid#69504PurposeExpresses AMPK alpha1 in mammalian cellsDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseRetroviralTagsHAExpressionMammalianPromoterSV40 early promoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Frankenbody-FLAG-mRuby2
Plasmid#175294PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mRuby2
TagsmRuby2ExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
M43: pHR-SFFVp-iLID::EGFP::FTH1
Plasmid#122149PurposeSelf-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.DepositorAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
TagsmStayGold(c4)ExpressionMammalianPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_GFP
Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralPromoterCaMKIIaAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIH-Myc-YAP-S94A
Plasmid#33094DepositorAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-H2B tdTomato
Plasmid#116870PurposeNuclear localized tdTomatoDepositorHas serviceAAV RetrogradeInsertH2B tdTomato
UseAAVAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-BRD4ΔN-mCherry-sspB
Plasmid#121968PurposeExpresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.DepositorInsertBRD4 (BRD4 Human)
UseLentiviralTagsmCherry-sspBExpressionMammalianMutationDeleted amino acids 1-461PromoterSFFVAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Luc-PS9
Plasmid#177942PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuc2 (ORF1ab )
ExpressionMammalianAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF1a_mCherry_P2A_Hygro_Barcode
Plasmid#120426PurposeBarcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertmCherry
UseLentiviralPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBUN411
Plasmid#50581PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserts3×FLAG-NLS-zCas9-NLS
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSMutationmaize codon optimizedPromoterOsU3p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEG BacMam C term 3C eGFP His8
Plasmid#160687PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTags3C eGFP His8ExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-PS9
Plasmid#177944PurposeTransient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP (ORF1ab )
ExpressionMammalianAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertVillin-mTQ2-P2A-FlpO-ERT2
TagsmTQ2, linked to FlpO through an P2A ribosomal ski…ExpressionMammalianPromoterVillinAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1/Ndi-1/myc-His
Plasmid#127503PurposeExpresses myc-tagged yeast Ndi-1 in mammalian cellsDepositorAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Green Lindoblum
Plasmid#163113PurposeExpresses biosensor for lactate in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGreen Lindolum
ExpressionMammalianPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpCas9 TadCBEd
Plasmid#193835PurposeExpress TadCBEd (with SpCas9) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTadA-CDd-SpCas9 D10A-2xUGI
ExpressionMammalianAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by