We narrowed to 12,103 results for: cel.2;

1. pBiFC-HA-hCaspase 2 FL (C303S)-VC155

   Plasmid

   #49257

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 (CASP2 Human)
   

   Use

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   C303S

   Promoter

   CMV

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pLenti-TRS-Leader-SARS-CoV-2-EGFP

   Plasmid

   #171585

   Purpose

   Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   TRS-Leader-SARS-CoV-2-d2eGFP
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

   Plasmid

   #49258

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 (CASP2 Human)
   

   Use

   Tags

   flag and split Venus N

   Expression

   Mammalian

   Mutation

   C303S

   Promoter

   CMV

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS SARS-CoV-2 BQ.1.1 Spike

   Plasmid

   #193710

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

   Insert

   SARS-CoV-2 BQ.1.1 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: T19I, LPPA24S, …

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   Nov. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pCAGGS SARS-CoV-2 BA.4.6 Spike

   Plasmid

   #190013

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

   Insert

   SARS-CoV-2 BA.4.6 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: T19I, LPPA24S, …

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   Sept. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCAGGS SARS-CoV-2 BA.2.75 Spike

   Plasmid

   #190012

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

   Insert

   SARS-CoV-2 BA.2.75 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the BA.2 mutations (T19I, LPPA24S, G142D…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   Sept. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCAGGS SARS-CoV-2 BA.1 Spike

   Plasmid

   #185452

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

   Depositor

   Marceline Côté

   Article

   Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

   Insert

   SARS-CoV-2 Spike BA.1 (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: A67V, Δ69-70, T…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCMV-GFP-CENPBpA-fixed-length-clone-2

   Plasmid

   #120180

   Purpose

   Randomly picked clone 2 of the pCMV-GFP-CENPBpA-fixed-length library

   Depositor

   David Bartel

   Article

   Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

   Insert

   CENPB poly(A) site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

    Plasmid

    #128760

    Purpose

    Expresses PARN gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PARN sgRNA #2 (PARN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

    Plasmid

    #128757

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.2 mir-1-1 reporter mir193b-2

    Plasmid

    #46732

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir193b-2 (MIR193B Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.2 mir-1-1 reporter mir50-2

    Plasmid

    #46711

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-2
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 mir-1-1 reporter mir44-2

    Plasmid

    #46705

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir44-2
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.2 mir-1-1 reporter mir30-2

    Plasmid

    #46689

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir30-2 (MIR30A Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.2 mir-1-1 reporter mir28-2

    Plasmid

    #46685

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir28-2 (MIR28 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

    Plasmid

    #128763

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #2 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03 TFAP2B

    Plasmid

    #67092

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP2B (TFAP2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 GTF2H2D

    Plasmid

    #67081

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H2D (GTF2H2C_2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only