Addgene

We narrowed to 12,812 results for: sequence

Showing: 801 - 820 of 12812 results

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EGFP-mOrange pHis-//

Plasmid

#53302

Purpose

Expresses His-tagged EGFP-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

EGFP

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pSaI20

Plasmid

#8615

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI20

Use

Tags

Expression

Bacterial

Mutation

N terminal threnine changed to alanine

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-SV40 pA Sense

Plasmid

#60643

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPTK007-3a-αMFΔ

Plasmid

#84966

Purpose

Type 3a, α-mating factor pre-sequence shortened - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor, pre-sequence shortened secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Smad4-MH1

Plasmid

#16492

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad4 (SMAD4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-276 of Smad4

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pKJ-1

Plasmid

#11333

Depositor

Michael McBurney

Article

Adra et al Gene. 1987 . 60(1):65-74.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Article

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pGL3con-miR15aBul

Plasmid

#21117

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_PGKY_APikL3

Plasmid

#123652

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

PGKY_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_CD156_APikL3

Plasmid

#123651

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

CD156_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR32_APikL2

Plasmid

#123649

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_APikL2

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR32_APikL3

Plasmid

#123650

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_APikL3

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGC4785

Plasmid

#113409

Purpose

Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

359_31121113_2

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3_ctrl_CP-candidate_luc+

Plasmid

#86392

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR143Bul

Plasmid

#21119

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-143 sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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p35SZ

Plasmid

#51513

Purpose

pMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
3kb filler sequence

Use

Plant expression t-dna

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pFP125

Plasmid

#21674

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been completely dele…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pLenti Puro HA-Ubiquitin-KO

Plasmid

#236292

Purpose

Lentiviral vector expressing HA-tagged ubiquitin KO mutant

Depositor

Addgene Research Program, Melina Fan

Insert

Ubiquitin KO (UBB Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Single ubiquitin coding sequence containing KO mu…

Promoter

CMV

Available since

April 15, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti Puro HA-Ubiquitin-K48

Plasmid

#236290

Purpose

Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

Depositor

Addgene Research Program, Melina Fan

Insert

Ubiquitin K48 (UBB Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Single ubiquitin coding sequence containing K48 m…

Promoter

CMV

Available since

April 10, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti Puro HA-Ubiquitin-K63

Plasmid

#236291

Purpose

Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

Depositor

Addgene Research Program, Melina Fan

Insert

Ubiquitin K63 (UBB Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Single ubiquitin coding sequence containing K63 m…

Promoter

CMV

Available since

April 10, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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