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pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-CDreV

Plasmid

#140133

Purpose

can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFKBIB_WT

Plasmid

#82219

Purpose

Gateway Donor vector containing NFKBIB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFKBIB (NFKBIB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WNT2_WT

Plasmid

#81775

Purpose

Gateway Donor vector containing WNT2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNT2 (WNT2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86020

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine, threonine 230 to al…

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SV2C_WT

Plasmid

#81924

Purpose

Gateway Donor vector containing SV2C, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SV2C (SV2C Human)

Use

Gateway entry vector

Mutation

F346S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)

Plasmid

#29438

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.) E653Q

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

Plasmid

#29446

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 520-1556 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pJC-elav1.8kb-TALE1-VP64-P10

Plasmid

#104609

Purpose

Expresses TALE1 under the control of a 1.8kb elav enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.8kb elav enhancer element (elav Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2gamma

Plasmid

#124145

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

Plasmid

#29439

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) with Q1116K mutation

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)

Plasmid

#29437

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) E653Q

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223_TPTE_WT

Plasmid

#81867

Purpose

Gateway Donor vector containing TPTE , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TPTE (TPTE Human)

Use

Gateway entry vector

Mutation

L452P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1alpha

Plasmid

#124139

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

Plasmid

#188770

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

Plasmid

#169467

Purpose

Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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ECON

Plasmid

#82553

Purpose

Epigenetic effector construct for targeted methylation

Depositor

Joseph Irudayaraj

Article

Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)

Tags

HALO and mCherry

Expression

Mammalian

Mutation

N-terminal photolyase homology region domain of C…

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLL5.0 ShCavin1-EGFP

Plasmid

#187253

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

Insert

ShRNA for Cavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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TMEM138-mCherry

Plasmid

#41630

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 138 (TMEM138 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only