We narrowed to 64,724 results for: cell;

1. pPN186

   Plasmid

   #91573

   Purpose

   Express sgRNA targeting human AKT3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   AKT3 (AKT3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80065

   Purpose

   Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Zic2a enhancer driving c-fos promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPN187

   Plasmid

   #91574

   Purpose

   Express sgRNA targeting human AKT3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   AKT3 (AKT3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIBN-TetR-tagRFP-T

   Plasmid

   #103809

   Purpose

   'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-TetR-tagRFP-T (CIB1, tetR E. coli, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79889

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-Foxo1-ADA 6KR

   Plasmid

   #17564

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1-ADA 6kr (Foxo1 Mouse)
   

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   K242, K245, K259, K262, K271 and K291 were replac…

   Promoter

   CMV

   Available Since

   Oct. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pMT-myl7-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80061

   Purpose

   Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Myl7 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-FKBP-TPD53

   Plasmid

   #172457

   Purpose

   Expression of Tumor Protein D52 like 1 (TPD53/TPD52L1) with N-terminal mCherry-FKBP tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   TPD53 (TPD52L1 Human)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5FRTTO-NHASt-codoptUL23

   Plasmid

   #135104

   Purpose

   Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL23 codon optimized
   

   Tags

   HA-Strep-Strep (N terminal on backbone)

   Expression

   Mammalian

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRTTO-codoptUL23-CStHA

    Plasmid

    #135099

    Purpose

    Codon-optimized HSV-1 UL23 with C-terminal StrepHA tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL23 codon optimized
    

    Tags

    Strep-Strep-HA

    Expression

    Mammalian

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-EYFP-hNCL

    Plasmid

    #103806

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hNCL (CIB1, NCL Mustard Weed, Human)
    

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTB2-RanGAP-Cerulean-Avi

    Plasmid

    #80068

    Purpose

    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Zebrafish biotagging

    Tags

    Avi

    Expression

    Bacterial

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

    Plasmid

    #62978

    Purpose

    Expresses myc-tagged mouse βTrCP residues 292-569

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codes for residues 292-569

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGEX4T-1 hSlp4-a del.SHD

    Plasmid

    #40067

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a V21A (SYTL4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted SHD domain (1-143)

    Promoter

    Tac

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pMIR-Report-Luc-KLF4-K1S

    Plasmid

    #62979

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Mutation

    Fragment 1-446 of KLF4

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pTet-O-ATOH7-T2A-PuroR

    Plasmid

    #162343

    Purpose

    Lentiviral expression of ATOH7 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ATOH7 (ATOH7 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR v2-sgDnmt1_1

    Plasmid

    #138347

    Purpose

    Express guide RNA 1 for Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR v2-sgDnmt1_2

    Plasmid

    #138348

    Purpose

    Express guide RNA 2 for mouse Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF-HA-TSAd-d239-74

    Plasmid

    #84560

    Purpose

    mammalian expression of TSAd-d239-74

    Depositor

    Anne Spurkland

    Article

    Berge et al PLoS One. 2010 Mar 18;5(3):e9761. doi: 1

    Insert

    TSAd (SH2D2A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of aa 239-274

    Promoter

    EF1a

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. EPHB4-gRNA1/2

    Plasmid

    #139974

    Purpose

    gRNA used for knockin EGFP into EPHB4 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    EPHB4 gRNA
    

    Expression

    Mammalian

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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