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pET-Trx-mSA2

Plasmid

#52320

Purpose

Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Depositor

Sheldon Park

Article

Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

Insert

monomeric streptavidin 2

Tags

FLAG, His, S tag, TEV site, and Thioredoxin

Expression

Bacterial

Mutation

S25H mutation compared to streptavidin

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

BFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBlu/gRNA

Plasmid

#59188

Purpose

Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Guide RNA Cassette

Use

CRISPR

Expression

Plant

Promoter

U6 (Arabidopis)

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pML107-PMR1

Plasmid

#226264

Purpose

Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

PMR1 gRNA (PMR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFPTET1CD

Plasmid

#82561

Purpose

Expression vector for antibody-sfGFP-TET1CD.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

scFvGCN4sfGFPTET1CD

Expression

Mammalian

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCGN-SRF

Plasmid

#11977

Purpose

For mammalian expression of human SRF with an HA tag

Depositor

Ron Prywes

Article

Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

Insert

SRF (SRF Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Tags

6X-His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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scAAV-hSyn-Cre

Plasmid

#138253

Purpose

A self-complementary AAV vector expresses Cre recombinase from hSyn promoter.

Depositor

Li Zhang

Article

Zingg et al J Neurosci. 2020 Apr 15;40(16):3250-3267

Insert

Cre recombinase

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (no Cre)

Plasmid

#200370

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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Clover-Geminin(1-110)

Plasmid

#83915

Purpose

Fluorescent probe for M/G1 transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pADV-Sec61b-TurboID

Plasmid

#179143

Purpose

Recombinant adenoviral plasmid for Sec61b-TurboID expression

Depositor

Jae Myoung Suh

Article

Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

Insert

Sec61b-V5-TurboID

Use

Adenoviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Tags

DsbC-6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAM5187

Plasmid

#120086

Purpose

Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid.

Depositor

Susan Golden

Article

Chen et al Microbiology. 2016 Dec;162(12):2029-2041

Type

Empty backbone

Use

Shuttle vector

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Article

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Insert

FUCCI

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_WT

Plasmid

#83482

Purpose

Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD2

Plasmid

#68161

Purpose

Universal domesticator plasmid for level 0 parts

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHD-DsRed

Plasmid

#51434

Purpose

Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

Depositor

Kate O'Connor-Giles

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

LoxP-3xP3-DsRed-LoxP

Promoter

3xP3

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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