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pVD1 Multiplexing Edit E4 (GB2241)

Plasmid

#160563

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E4)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PI3KC2alpha-PX (1405-1545)

Plasmid

#119119

Purpose

Bacterial expression of human phox homology (PX) domain, PI3KC2alpha-PX (1405-1545)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

PI3KC2alpha-PX (1405-1545) (PIK3C2A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-GFP-PGK-Puro

Plasmid

#110865

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCBS-5740

Plasmid

#249327

Purpose

Expresses sgRNA targeting the fcy gene in S. cerevisiae

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

sgRNA targeting fcy

Expression

Yeast

Mutation

N/A

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5737

Plasmid

#249326

Purpose

Expresses Non-targeting sgRNA

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

nontargeting sgRNA

Expression

Yeast

Mutation

N/A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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M55

Plasmid

#242623

Purpose

Strep-Flag-MYCMutMBIV-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-Flag-MYCMutMBIV-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

A310E,A311D,P312G,P313G,R315A,K316A,D317S

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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M54

Plasmid

#242622

Purpose

Strep-Flag-MYCMutMBIIIa-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-Flag-MYCMutMBIIIa-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

I189A,V193A,V194A,F195S,L199A

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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M53

Plasmid

#242621

Purpose

Strep-Flag-MYCMutMBII-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-Flag-MYCMutMBII-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

I128A,I129S,I130A,M134A,F137A

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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M24

Plasmid

#242619

Purpose

Strep- MYC deltaCTD, deltaMB0-1-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep- MYC deltaCTD, deltaMB0-1-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

delta2-62, delta354-439

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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M20

Plasmid

#242618

Purpose

Strep-MYC deltaCTD-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-MYC deltaCTD-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Mutation

delta354-439

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe-Hygro-RAS

Plasmid

#242639

Purpose

RAS expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

HRASG12V (HRAS Human)

Expression

Mammalian

Mutation

G12V

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M91

Plasmid

#242635

Purpose

lower MYCdeltaCTD expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYCdeltaCTD (MYC Human)

Tags

V5

Expression

Mammalian

Mutation

delta354-439

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M99

Plasmid

#242638

Purpose

MYC knockdown + rescue

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIIIa (MYC Human)

Use

RNAi

Tags

V5

Mutation

I189A,V193A,V194A,F195S,L199A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M76B

Plasmid

#242628

Purpose

MYCMutMBIV expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIV (MYC Human)

Tags

V5

Expression

Mammalian

Mutation

A310E,A311D,P312G,P313G,R315A,K316A,D317S

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M77

Plasmid

#242629

Purpose

MYC knockdown + rescue

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIIIa, MutMBIV (MYC Human)

Use

RNAi

Tags

V5

Mutation

I189A,V193A,V194A,F195S,L199A,A310E,A311D,P312G,P…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M77B

Plasmid

#242630

Purpose

MYCMutMBIIIa expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIIIa (MYC Human)

Use

RNAi

Tags

V5

Mutation

I189A,V193A,V194A,F195S,L199A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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