161,472 results

1. pLenti-hACE2-hygro

   Plasmid

   #161758

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCMV_NESPylRS(AF)_hU6tRNAPyl

   Plasmid

   #223508

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y306A; Y384F

   Promoter

   CVM, SP6 and U6

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIM33-GFP

   Plasmid

   #65399

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   TRIM33 (TRIM33 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 HA Arf6 DN T27N

   Plasmid

   #10831

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 T27N (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T27N. Defective in GTP binding. Dominant negati…

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-BCR-ABL-p190-FLAG

   Plasmid

   #205620

   Purpose

   Expression of BCR-ABL oncogene in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

   Insert

   BCR-ABL oncogene, p190 isoform b3a2
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T117M- Please see depositor comments

   Promoter

   CMV

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAF112

   Plasmid

   #135920

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Luciferase; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK444 (EF1-OsTIR1(F74G) Puro)

   Plasmid

   #214374

   Purpose

   EF1-OsTIR1(F74G) (PiggyBac)

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   EF1-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBC-MT1T2

   Plasmid

   #50593

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T1, gRNA scaffold, OsU3t plus, TaU3p, T2
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BE2

   Plasmid

   #73020

   Purpose

   Expresses BE2 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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