We narrowed to 2,492 results for: MEA
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Plasmid#167478PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(I539E)-ShadowG
TagsShadowG and mEGFP(A206K)ExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG
Plasmid#167479PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(C450A)-ShadowG
TagsShadowG and mEGFP(A206K)ExpressionMammalianPromoterCMVAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 2
Plasmid#99317PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorExpressionMammalianAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ssp3
Plasmid#86382PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ssp3 enhancer
UseStap-seq screening vectorAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ham
Plasmid#86383PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ham enhancer
UseStap-seq screening vectorAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-sgl
Plasmid#86384PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster sgl enhancer
UseStap-seq screening vectorAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-ncm
Plasmid#86385PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster ncm enhancer
UseStap-seq screening vectorAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFAB806
Plasmid#47849PurposeBIOFAB reporter plasmid for measuring lambda tR2 termination efficiencyDepositorInsertlambda tR2 terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO4Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB805
Plasmid#47850PurposeBIOFAB reporter plasmid for measuring tonB termination efficiencyDepositorInserttonB terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO5Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB816
Plasmid#47851PurposeBIOFAB reporter plasmid for measuring ilvGEDA termination efficiencyDepositorInsertilvGEDA
UseSynthetic BiologyExpressionBacterialPromoterpLTetO6Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB807
Plasmid#47852PurposeBIOFAB reporter plasmid for measuring RNAI termination efficiencyDepositorInsertRNAI terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO7Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB820
Plasmid#47855PurposeBIOFAB reporter plasmid for measuring tetAC termination efficiencyDepositorInserttetAC terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO10Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB1910
Plasmid#47859PurposeBIOFAB reporter plasmid for measuring rpoC termination efficiencyDepositorInsertrpoC terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO14Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB804
Plasmid#47848PurposeBIOFAB reporter plasmid for measuring T3 early termination efficiencyDepositorInsertT3 early terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO3Available SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3905
Plasmid#47808PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.DepositorInsertpromoter 14 and BCD1
UseSynthetic BiologyExpressionBacterialPromotersee insertAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi1-91-NLuc
Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα15-244-NLuc
Plasmid#207154PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
TagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_G299C
Plasmid#177829PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_G299C
ExpressionMammalianMutationG299CPromoterCMVAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pyronic/pcDNA3.1(-)
Plasmid#51308PurposePyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.DepositorInsertPyronic
ExpressionMammalianPromoterCMVAvailable SinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657
Plasmid#212962PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_non-targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianPromoterU6Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657
Plasmid#212963PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_GFP targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianPromoterU6Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH21-BNA-Luc
Plasmid#187924PurposeExpresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cellsDepositorInsertReporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
ExpressionMammalianPromoterRNA polymease I PromoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TPC2-G-GECO1.2
Plasmid#207141PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channelDepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 WT
Plasmid#11024DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
SDHC-pHLuorin-sems
Plasmid#107767Purposelabeling of mitochondrial succinate dehydrogenase subunit C, pH measurementsDepositorInsertSubunit C of SDH fused to pHluorin (SDHC Human)
TagspHluorinExpressionMammalianPromoterCMVAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
HP35 tension sensor
Plasmid#101250PurposeExpresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.DepositorInsertYPet(short)-HP35-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianPromoterCMVAvailable SinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
ExpressionMammalianMutationA215CPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LVDP-CArG-RE-GPR
Plasmid#89762PurposeQuantitative measurement of CArG response element activity.DepositorInsert6X CArG-RE
UseLentiviralTagsCMV-ZsGreenExpressionMammalianAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-pSu9-HaloTag7-SNAP
Plasmid#217319PurposeExpresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.DepositorInsertNeurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
UseRetroviralTagsHaloTag and SNAPPromoterCMVAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
ExpressionMammalianMutationC289TPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
ExpressionMammalianMutationA191GPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
F40 tension sensor
Plasmid#101252PurposeExpresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.DepositorInsertYPet(short)-F40-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianPromoterCMVAvailable SinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
scAAV-Mlc2v-MPRA
Plasmid#182649PurposeSelf complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assayDepositorInsertmCherry
UseAAVExpressionMammalianPromoterrat Mlc2vAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlmRFP1
Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromoterJ23100Available SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
stabilized HP35 tension sensor module
Plasmid#101251PurposeExpresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.DepositorInsertYPet(short)-HP35st-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianPromoterCMVAvailable SinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFAB3677
Plasmid#47833PurposeBIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.DepositorInsertpromoter 2 and BCD1
UseSynthetic BiologyExpressionBacterialPromotersee insertAvailable SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3833
Plasmid#47839PurposeBIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.DepositorInsertpromoter 8 and BCD1
UseSynthetic BiologyExpressionBacterialPromotersee insertAvailable SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only