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pDEST 566-PHL2

Plasmid

#118676

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 566-XERICO

Plasmid

#118677

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rab26 in pCSDEST2

Plasmid

#140884

Purpose

Expression of zebrafish GFP-tagged Rab26 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab26 (rab26 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rab40b in pCSDEST2

Plasmid

#140883

Purpose

Expression of zebrafish GFP-tagged Rab40b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab40b (rab40b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rab13 in pCSDEST2

Plasmid

#140879

Purpose

Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab13 (rab13 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-CaaX(tH) in pCSDEST2

Plasmid

#140890

Purpose

Expression of GFP-CaaX (sequence from Hras) in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX membrane targeting motif (H-ras)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-DEST-R1R4

Plasmid

#141054

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-DEST-R5R4

Plasmid

#141055

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-2XFL-Src-S4-UD

Plasmid

#140305

Purpose

Expresses Flag-tagged human Src-S4-UD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH4 and UD regions

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-2XFL-Src-S4-UD-KD

Plasmid

#140301

Purpose

Expresses Flag-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

lacks the SH3 and SH2 domains

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-flox-R4R3

Plasmid

#139517

Purpose

Provides two loxP sequences between gene cassettes in the destination vector

Depositor

Minoru Ko

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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JK104: pMVP-DEST

Plasmid

#121851

Purpose

pMVP destination vector, empty expression plasmid backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-DEST51 PNRC1

Plasmid

#123293

Purpose

Expression of PNRC1 in mammalian cells

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CMYA5tnC/pDEST26-N-FLAG

Plasmid

#98130

Purpose

Express CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector

Depositor

Rita Shiang

Insert

CMYA5 (CMYA5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CMYA5tnT/pDEST26-N-FLAG

Plasmid

#98131

Purpose

Express CMYA5 (aa 3859 – 4069) Leu allele (4063) with a N-terminal FLAG tag in pDEST26 mammalian expression vector

Depositor

Rita Shiang

Insert

CMYA5 (CMYA5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

rs10043986 (NM_153610.4:c.12188C>T)

Promoter

CMV

Available since

Aug. 3, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEJS427-pCSDest2-AcrE2

Plasmid

#85677

Purpose

Mammalian expression of anti-CRISPR protein from Type I-E

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type I-E anti-CRISPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARL4-V5-His6

Plasmid

#67440

Purpose

Mammalian expression of hArl4 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 105 A to T; silent mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL5A-V5-His6

Plasmid

#67441

Purpose

Mammalian expression of hArl5 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL5B-V5-His6

Plasmid

#67442

Purpose

Mammalian expression of hArl5b with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5B (ARL5B Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL4C-V5-His6

Plasmid

#67444

Purpose

Mammalian expression of hArl4c with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL4D-V5-His6

Plasmid

#67447

Purpose

Mammalian expression of hArl4D with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL14-V5-His6

Plasmid

#67448

Purpose

Mammalian expression of hArl14 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL11-V5-His6

Plasmid

#67449

Purpose

Mammalian expression of hArl11 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

bp 442 T to C; aa C148R

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARFRP1-V5-His6

Plasmid

#67450

Purpose

Mammalian expression of hArfrp with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF3-GFP

Plasmid

#67391

Purpose

Mammalian expression of hArf3 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF5-GFP

Plasmid

#67393

Purpose

Mammalian expression of hArf5 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4D-GFP

Plasmid

#67405

Purpose

Mammalian expression of hArl4D with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST15-GST-C11orf83

Plasmid

#65848

Purpose

bacterial expression of GST (N-ter) - C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

WT

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS321-pDestMyc-USP44catDead

Plasmid

#43902

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

Expression

Mammalian

Mutation

C282A

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pSCT-GAL93-DEST

Plasmid

#46339

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Type

Empty backbone

Use

Tags

6XHIS

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81

Plasmid

#45293

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

deleted 81 N-terminal amino acids

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaC27

Plasmid

#45292

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

deleted 27 c-terminal amino acids

Promoter

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN149

Plasmid

#45294

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
alpha 0-CFP in pdest12.2

Plasmid

#36312

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

Introduced KpnI and SpeI sites between 92nd and 9…

Promoter

CMV

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pETCON-HB28/design_25

Plasmid

#30260

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB28

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-E43/design_55

Plasmid

#30237

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

Mtb ACP2 designed binding protein E43

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB14/design_53

Plasmid

#30249

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB14

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB50/design_43

Plasmid

#30270

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB50

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB56/design_34

Plasmid

#30276

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB56

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB4/design_2

Plasmid

#30271

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 16, 2011

Availability

Academic Institutions and Nonprofits only

Showing: 1641 - 1680 of 152226 results

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