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Showing: 2051 - 2100 of 15391 results

  2. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  3. pTT3-ecdICAM1-ST3-His

    Plasmid
    #210666
    Purpose
    Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210666-plasmid-map-sequence-id-417657

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  4. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

    Plasmid
    #186740
    Purpose
    Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene
    Depositor
    Richard Sherwood
    Article
    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.
    Insert
    RAB10, RABIF (RAB10, RABIF Human)
    Use
    Lentiviral
    Tags
    RAB10-T2A-RABIF-P2A-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    186740-plasmid-map-sequence-id-389283

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  5. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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  7. pTT3-ecdCD86-ST3-His

    Plasmid
    #210664
    Purpose
    Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210664-plasmid-map-sequence-id-417658

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  8. pTT3-ecdCD80-ST3-His

    Plasmid
    #210665
    Purpose
    Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210665-plasmid-map-sequence-id-443131

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  12. Capping Protein

    Plasmid
    #89950
    Purpose
    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.
    Depositor
    Guillaume Romet-Lemonne, Antoine Jégou
    Article
    Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.
    Inserts
    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    Use
    Tags
    6*His
    Expression
    Bacterial
    Mutation
    V222I
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89950-plasmid-map-sequence-id-183281

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  22. pAAV_BiPVe4_ChR2_mCherry

    Plasmid
    #213937
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213937-plasmid-map-sequence-id-421852

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  30. pAAV_BiPVe3_ChR2_mCherry

    Plasmid
    #213941
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213941-plasmid-map-sequence-id-421864

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  31. pAAV_BiCHATe27_ChR2_mCherry

    Plasmid
    #213830
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213830-plasmid-map-sequence-id-421862

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  34. pAAV_BiVIPe4_ChR2_mCherry

    Plasmid
    #213856
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213856-plasmid-map-sequence-id-421843

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  35. pAAV_BiSSTe4_ChR2_mCherry

    Plasmid
    #213945
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213945-plasmid-map-sequence-id-421869

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  39. pAAV_BiSSTe10_ChR2_mCherry

    Plasmid
    #213815
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213815-plasmid-map-sequence-id-421845

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  42. pAAV_BiLAMP5e3_ChR2_mCherry

    Plasmid
    #213915
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213915-plasmid-map-sequence-id-421865

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  47. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Samie Jaffrey
    Article
    Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    102277-plasmid-map-sequence-id-197159

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  50. CbetaS-pA

    Plasmid
    #32104
    Depositor
    Michael Cole, Yardena Samuels
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Sept. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 2051 - 2100 of 15391 results