We narrowed to 839 results for: Hr

1. pIRES-hrGFP II-mTET1

   Plasmid

   #83569

   Purpose

   Expresses TET1 with a mutated catalytic domain in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   catalytic domain mutant H1672D, D1674A

   Promoter

   CMV

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBUN501

   Plasmid

   #50582

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   zCas9D10A
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Cas9D10A nickase, was derived from the zCas9 and …

   Promoter

   AtU6-26p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_HGS_VHS

   Plasmid

   #109890

   Purpose

   Protein expression and purification of HGS_VHS

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   HGS_VHS (HGS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-guide-puro mHgs-1

   Plasmid

   #198481

   Purpose

   lentiviral stable expression of mHgs gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mHgs (Hgs Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-guide-puro mHgs-2

   Plasmid

   #198482

   Purpose

   lentiviral stable expression of mHgs gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mHgs (Hgs Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ID1GFP Donor

   Plasmid

   #119282

   Purpose

   Mouse ID1-eGFP for HR to create reporter

   Depositor

   Joan Massague

   Article

   Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

   Insert

   Id1GFP + homology arms (Id1 Mouse)
   

   Use

   Reporter

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJOP-HTT-HR18Q

   Plasmid

   #87228

   Purpose

   HTT HR arms with 18 CAG repeats and piggyBac selection cassette

   Depositor

   Mahmoud Pouladi

   Article

   Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

   Insert

   1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a driving selection cassette

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-W

   Plasmid

   #105032

   Purpose

   Lentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   gRNA targeting Hgs (Hgs Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. C321.ΔA.exp

   Bacterial Strain

   #49018

   Purpose

   This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

   Depositor

   George Church

   Article

   Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. C321.ΔA

    Bacterial Strain

    #48998

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

11. C321

    Bacterial Strain

    #48999

    Purpose

    Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

12. C321.deltaA (Isaacs lab)

    Bacterial Strain

    #73581

    Purpose

    Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Bacterial Resistance

    Ampicillin

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pD649-HAsp-PTPRM-COMP5AP-AviTag-9xHis

    Plasmid

    #157519

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PTPRM (PTPRM Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD649-HAsp-PTPRM-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156955

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PTPRM (PTPRM Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. BW-Para

    Bacterial Strain

    #172602

    Purpose

    The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Bacterial Resistance

    None

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pJOP-HTT-HR65Q

    Plasmid

    #92249

    Purpose

    HTT donor DNA with 65Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOP-HTT-HR81Q

    Plasmid

    #92250

    Purpose

    HTT donor DNA with 81Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJOP-HTT-HR30Q

    Plasmid

    #92247

    Purpose

    HTT donor DNA with 30Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJOP-HTT-HR45Q

    Plasmid

    #92248

    Purpose

    HTT donor DNA with 45Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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