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Plasmid#226793PurposeIntegrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10BDepositorInsertslr0214 (slr0214 Synechocystis sp. PCC 6803)
UseTagsExpressionBacterialMutationPromoterTetracycline resistance cassette promoter (consti…Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
T2A 3xNLS GFP
Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5417_p3WJdB_promoter dropout vector
Plasmid#221191PurposePromoter probe vector with dropout sequence for promoter sequences with 3WJdB reporterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterPromoter Placeholder/Dropout VectorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP578
Plasmid#222957PurposeExpresses R144S LoaPDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 144 to SerinePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP577
Plasmid#222956PurposeExpresses K142S LoaPDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Lysine 142 to SerinePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP576
Plasmid#222955PurposeExpresses R141S LoaPDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 141 to SerinePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP575
Plasmid#222954PurposeExpresses R36S LoaPDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 36 to SerinePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP573
Plasmid#222952PurposeExpresses E40H LoaPDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Glutamate 40 to HistidinePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MARS-nGFP
Plasmid#205234PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-2xMARS-PLD
Plasmid#205237PurposeExpresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMFDepositorInsertsUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP572
Plasmid#222951PurposeExpresses E40A LoaPDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Glutamate 40 to AlaninePromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV02
Plasmid#212156PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.DepositorInsertsecondary alcohol dehydrogenase
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHFdist_rm
Plasmid#162318PurposeP. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removedDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHF distal site is removedPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBZS-YP_001498439
Plasmid#198370PurposeExpress YP_001498439 in bacterial cells.DepositorInsertYP_001498439
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37U
Plasmid#196659PurposePlasmid for overexpression of mutated initiator tRNA (A37U)DepositorInserttRNAfMet-A37U expression cassette
UseTagsExpressionBacterialMutationA37U in tRNAfMetPromotermetY native promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37G
Plasmid#196658PurposePlasmid for overexpression of mutated initiator tRNA (A37G)DepositorInserttRNAfMet-A37G expression cassette
UseTagsExpressionBacterialMutationA37G in tRNAfMetPromotermetY native promoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENT L1-Kozak-ERK-KTR-L2
Plasmid#186353PurposeEntry clone with ORF encoding ERK KTR flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertERK Kinase Translocation Reporter (MAPK1 Synthetic, Human)
UseProtein expressionTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-luc-miR-124
Plasmid#171558Purposea positive control reporter with 3' UTR sequences perfectly complementary to miR-124DepositorInserta sequences perfectly complementary to miR-124
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-luc-miR-1
Plasmid#171556Purposea positive control reporter with 3' UTR sequences perfectly complementary to miR-1DepositorInserta sequences perfectly complementary to miR-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
C238-E07: attB2r-SV40polyA-attB3
Plasmid#162917PurposeGateway attB2r/attB3 entry clone containing a polyA sequence for transcription terminationDepositorInsertSV40 polyadenylation sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only