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pGEX-4T1-Cdc42 G15A

Plasmid

#69356

Purpose

expression of nucleotide free Cdc42 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-UAS-mIFNγ

Plasmid

#230004

Purpose

Gal4-dependent expression of secreted mouse IFNγ

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

UAS-mIFNγ (Ifng Synthetic)

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Craf R401H

Plasmid

#118334

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf R401H (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R401H

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-SUMO-RNF168ring (1-189)

Plasmid

#63570

Purpose

Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

RNF168 (RNF168 Human)

Tags

6xHis and SUMO3

Expression

Bacterial

Mutation

Ring domain only (residues 1-189)

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-Rheb(5A)

Plasmid

#192439

Purpose

Encodes mCherry-tagged Rheb lacking residues 38-42.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb amino acids 38-42 deleted.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mcherry-Myo 9b R1695M

Plasmid

#134958

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-M2-PAK2 wt

Plasmid

#31680

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Rac1 G15A

Plasmid

#69355

Purpose

expression of nucleotide free Rac1 in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

Plasmid

#101351

Purpose

HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVXT-mWnt3

Plasmid

#121934

Purpose

doxycycline-inducible mouse Wnt3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Wnt3 (Wnt3 Mouse)

Use

Lentiviral

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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p7274 MSCV-P C-FlagHA 16 ∆DLYC

Plasmid

#163304

Purpose

Expresses HPV16 E7 ∆DLYC

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 ∆DLYC

Use

Retroviral

Tags

FlagHA

Mutation

∆DLYC

Promoter

MSCV LTR

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUb-tdTomato-LP-Neo-LP

Plasmid

#231509

Purpose

Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

tdTomato fluorescent protein

Use

Unspecified; Acanthamoeba castellanii neff strain

Promoter

Acanthamoeba polyubiquitin

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGAPDH-Cre-HYG

Plasmid

#231510

Purpose

Expresses Cre recombinase in Acanthamoeba

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

Cre recombinase

Use

Unspecified; Acanthamoeba castellanii neff strain

Tags

EGFP and SV40 NLS

Promoter

Acanthamoeba GAPDH

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-ARF

Plasmid

#19929

Depositor

Yue Xiong

Article

Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

Insert

cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)

Expression

Bacterial

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pCS2 hSmad1-GM

Plasmid

#22992

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S210A ,T202A, S191A, S198A

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
KG#203

Plasmid

#110880

Purpose

Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-Craf SSDD

Plasmid

#118337

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf SSDD (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

SSDD

Promoter

CMV

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHS1398

Plasmid

#205971

Purpose

MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL N-Myc-hPOT1_deltaOB

Plasmid

#166408

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Tags

myc

Expression

Mammalian

Mutation

ΔOB

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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