We narrowed to 15,943 results for: genome
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pYLsgRNA-AtU3d/LacZ
Plasmid#66201Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterAtU3dAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTRW22K_7Ti1
Plasmid#177293Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZDepositorInsertlacZ
UseSynthetic Biology; Yeast expression, rrn genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-48xH3C1 array
Plasmid#236384PurposeExpression of array of 48 crRNAs targeting H3C1DepositorInsert48xH3C1 crRNA array (H3C1 Human)
UseCRISPR and LentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)
Plasmid#133794PurposeU6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018DepositorInsertNmeCas9 and Nme2Cas9 sgRNA entry vector
ExpressionMammalianMutationsgRNA architecture from Amrani et al. Genome Biol…PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_EF1aUBA3_IRES_Puro
Plasmid#235660Purposehuman UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)DepositorAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-Cas9n-Act3.0
Plasmid#178956PurposeIt consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
scaffold-linker-U6
Plasmid#89639Purposeconstruction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterhU6Available SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-1
Plasmid#72680PurposeSame as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationlower strength RBS before mutL E32K allele than i…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-POLD3
Plasmid#183194PurposeExpression cloneDepositorInsertPOLD3
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA184
Plasmid#216047PurposeFragmid fragment: (C' terminus) for prime editing; using PEmaxDepositorHas ServiceCloning Grade DNAInsertSV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA063
Plasmid#216020PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9-SpRY (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA061
Plasmid#216018PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9-NG (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA183
Plasmid#216025PurposeFragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiencyDepositorHas ServiceCloning Grade DNAInsertnCas9 (H840A)_v2.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA062
Plasmid#216019PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9-SpG (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA060
Plasmid#216017PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorHas ServiceCloning Grade DNAInsertnCas9 (H840A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/RFP-DDX54
Plasmid#97061PurposeExpresses RFP (tdTomato)-tagged DDX54 in mammalian cellsDepositorAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Syt7 KI
Plasmid#131488PurposeEndogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)DepositorAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJGL001
Plasmid#180605PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasXDepositorInsertDpbCasX
UseCRISPRTags10x His and MBPExpressionBacterialPromoterT7Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only
