Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,943 results for: genome

Clear keywords
Showing: 8401 - 8420 of 15943 results

  3. pNOC_hfnCas12a_CRATES

    Plasmid
    #176251
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176251-plasmid-map-sequence-id-347017

    Enlarge

  8. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

    Enlarge

  10. pAS_4xEcoLeuT(CUA)_AnapRS

    Plasmid
    #140019
    Purpose
    Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    EcoLeuRS (AnapRS)
    Expression
    Mammalian
    Mutation
    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…
    Promoter
    EF1
    Available Since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140019-plasmid-map-sequence-id-297114

    Enlarge

  13. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140447-plasmid-map-sequence-id-296176

    Enlarge

  16. pRECMYC-Ebu1

    Plasmid
    #240713
    Purpose
    pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ebu1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240713-plasmid-map-sequence-id-482476

    Enlarge

  18. PB-XL-AncBE4max

    Plasmid
    #136254
    Purpose
    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max
    Depositor
    Zhaohui Ye
    Article
    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:
    Insert
    AncBE4max_GFP
    Use
    CRISPR; Piggybac dna transposon
    Expression
    Mammalian
    Mutation
    The AncBE4max_GFP insert was PCR amplified from a…
    Promoter
    TRE3G
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136254-plasmid-map-sequence-id-270570

    Enlarge

Showing: 8401 - 8420 of 15943 results