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MSCV-miR-34a

Plasmid

#63932

Purpose

MSCV vector for overexpressing mouse miR-34

Depositor

Lin He

Article

Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

Insert

microRNA 34a (Mir34a Mouse)

Expression

Mammalian

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pVSVG-PATagRFP

Plasmid

#31947

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

VSVG

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Ms

Plasmid

#61231

Purpose

Lentivirus expressing shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (Stk11 Human, Mouse)

Use

Lentiviral

Promoter

H1/TO

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-FAS-TdTomato-WPRE-pA

Plasmid

#37092

Purpose

Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pKMD106e - intein-Cas9(S219)

Plasmid

#64190

Purpose

Expresses intein-Cas9(S219) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-fulllength

Plasmid

#46076

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-fulllength (Map7 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET-(-30)GFP-9xGGS-Cre-6xHis

Plasmid

#62372

Purpose

Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP-9xGGS-Cre-6xHis

Tags

(-30)GFP and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Expression

Bacterial

Available Since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pNCS-mCardinal2

Plasmid

#52631

Purpose

bacterial expression vector with far-red FP mCardinal2

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mCardinal2

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only

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anti-Notch2_B6-pBIOCAM5

Plasmid

#39347

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Tags

GFP

Expression

Worm

Promoter

meg-3

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
3122 pSFFV-neo Bcl-XL MI-3

Plasmid

#8811

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-XL MI-3 (BCL2L1 Human)

Expression

Mammalian

Mutation

BH1 WGR-->WAR

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pMy-CTCF-GFP

Plasmid

#50574

Purpose

Retroviral vector endoding for mouse CTCF fused to GFP

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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3410 pBM272 Bcl-XL

Plasmid

#8772

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Expression

Yeast

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pENTR-FOXP2

Plasmid

#47053

Purpose

Expresses forkhead box P2

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Forkhead Box P2 (FOXP2 Human)

Use

Gateway entry vector

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pGL3 Pro E2

Plasmid

#48749

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing E-box2 (-112 to +98)

Use

Luciferase

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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PKG I alpha CAT

Plasmid

#16392

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, S65D

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pBJ1 neo-hu FceRI gamma

Plasmid

#16540

Depositor

Jean Kinet

Article

Kuster et al J Biol Chem 1990 Apr 15;265(11):6448-52.

Insert

FCER1G (FCER1G Human)

Expression

Mammalian

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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