We narrowed to 58,593 results for: LIS

1. pYPQAT37

   Plasmid

   #223409

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT40

   Plasmid

   #223412

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGKpuro

   Plasmid

   #11349

   Depositor

   Rudolf Jaenisch

   Article

   Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

   Plasmid

   #132406

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19/N-mChe

   Plasmid

   #183159

   Purpose

   Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-DeltaJunD

   Plasmid

   #68547

   Purpose

   AAV expression of DeltaJunD

   Depositor

   Eric Nestler

   Article

   Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

   Insert

   DeltaJunD-IRES-GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   truncated splice variant

   Promoter

   CMV

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pTF102

   Plasmid

   #134774

   Purpose

   A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.

   Depositor

   Kan Wang

   Article

   Frame et al Plant Physiol. 2002 May;129(1):13-22. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV sgRNA Expression Plasmid

   Plasmid

   #174540

   Purpose

   Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

   Depositor

   Ormond MacDougald

   Article

   Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Lyn-dInPAkt(R23/25A)

   Plasmid

   #181835

   Purpose

   Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-dInPAkt
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Mutation

   Arginines 23 and 25 in PH domain mutated to alani…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEMHE-mEGFP-mTrim21

    Plasmid

    #105519

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MiniCoopR mitfa:EGFP

    Plasmid

    #118850

    Purpose

    Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    mitfa: EGFP (mitfa Synthetic, Zebrafish)
    

    Use

    Zebrafish expression, tol6

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p10xQUAS-CsChrimson

    Plasmid

    #163629

    Purpose

    10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

    Depositor

    Christopher Potter

    Article

    Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

    Insert

    CsChrimson.mVenus
    

    Use

    Tags

    mVenus

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMVTAG-NEMO

    Plasmid

    #11970

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 5HT2A-APEX2

    Plasmid

    #215558

    Purpose

    Stable, inducible expression of 5HT2A

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-dCas9-MQ1(WT)-EGFP

    Plasmid

    #89633

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a - Ec-ClpX

    Plasmid

    #111516

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Flag-Craf R89L

    Plasmid

    #118333

    Purpose

    Expression of human Craf

    Depositor

    Philip Stork

    Article

    Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

    Insert

    Craf R89L (RAF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R89L

    Promoter

    CMV

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLv-CMV-MyoD-ER(T)

    Plasmid

    #26809

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD-ER(T) (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pPHT-PKA

    Plasmid

    #60936

    Purpose

    Fluorescent FPX sensor for cAMP

    Depositor

    Anne Marie Quinn

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    PKA FPX Sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBECKP-spe

    Plasmid

    #117236

    Purpose

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge