We narrowed to 16,367 results for: nans

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NES-EGFP-cPHx3

Plasmid

#116855

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-RARalpha

Plasmid

#35555

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DNAseI

Plasmid

#182409

Purpose

Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DNase I (DNASE1 Bovine)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT

Plasmid

#216413

Purpose

Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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Pseudojanin (PJ)

Plasmid

#37999

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pFLAG-Egfr(Velvet)

Plasmid

#18788

Depositor

Bruce Beutler

Article

Du et al Genetics. 2004 Jan . 166(1):331-40.

Insert

epidermal growth factor receptor (Egfr Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D833G; signal sequence removed (aa1-24)

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130999

Purpose

Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

Ef1a

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

Plasmid

#130998

Purpose

Double floxed ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

Ef1a

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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PM-RspA-CFAST

Plasmid

#233601

Purpose

Expression of RspA-CFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV INS2x mRFP-3MREs (AAV-beta)

Plasmid

#206928

Purpose

Beta cell-specific gene expression from AAV in human pancreatic islets

Depositor

Markus Grompe

Article

Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

Insert

mRFP

Use

AAV

Expression

Mammalian

Promoter

INSx2

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p190-FLAG

Plasmid

#205620

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p190 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

T117M- Please see depositor comments

Promoter

CMV

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRVdG-4Cre

Plasmid

#98034

Purpose

First-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-cPHx3

Plasmid

#116862

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCWori cytochrome P450-BM3h 9D7

Plasmid

#61308

Purpose

Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

Depositor

Alan Jasanoff

Article

Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

Insert

Cytochrome P450-BM3 heme domain 9D7

Tags

6xHis

Expression

Bacterial

Mutation

T268A, I263A, T438V, A328G

Promoter

Tac

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAT-YFP-TetR

Plasmid

#59018

Purpose

Expresses a fusion of yellow ﬂuorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

Depositor

David Sibley

Article

Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

Insert

YFP-TetR

Use

Toxoplasma expression

Promoter

alpha-tubulin

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTBK1

Plasmid

#87443

Purpose

express TBK1 in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

TBK1 (Tbk1 Mouse)

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-LSD1

Plasmid

#92362

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-LSD1

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO EYFP

Plasmid

#55641

Purpose

Flp-Dependent EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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