161,697 results

1. pCMV CEPIA3mt

   Plasmid

   #58219

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA3mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V E334D M339L

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. P427-FBP_13

   Plasmid

   #139704

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-HA-deLACCO1-NGR

   Plasmid

   #208024

   Purpose

   Control biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   deLACCO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPYtag-GFPnanobody

   Plasmid

   #214837

   Purpose

   This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-GFPnanobody
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CBE-T1.46

   Plasmid

   #193284

   Purpose

   Expression of CBE-T1.46 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.46
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-GFAP-hM4D(Gi)-mCherry

   Plasmid

   #50479

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

   Plasmid

   #209079

   Purpose

   Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   sfGFP
   NGN2 (Neurog2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV4 p65

   Plasmid

   #21966

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NFkB p65 subunit (RELA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   This construct was the product of 3 subcloning pr…

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCsRNAP

    Plasmid

    #182746

    Purpose

    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

    Depositor

    James Collins

    Article

    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

    Insert

    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pscALPSpuro-MmACE2 (Mouse)

    Plasmid

    #158087

    Purpose

    Expresses mouse ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Mouse ACE2 (Ace2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-G-GECO1.2

    Plasmid

    #32446

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGPA1

    Plasmid

    #115476

    Purpose

    Generation of mariner-based transposon mutant libraries in Enterococcus faecium

    Depositor

    Willem van Schaik

    Article

    Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

    Plasmid

    #105556

    Purpose

    AAV expression of EGFP from tMCK promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tMCK

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mini-donor - SOX17-EGFP

    Plasmid

    #206048

    Purpose

    this mini-donor knockin vector can be used to knock EGFP into SOX17 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Use

    Knockin donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p663-UBC-miniTurbo-V5-PRKACB_IDG-K

    Plasmid

    #170602

    Purpose

    Gateway destination clone of PRKACB (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-PRKACB (PRKACB Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6p1-hPdcd4

    Plasmid

    #20693

    Depositor

    Haiwei Song

    Article

    Loh et al EMBO J. 2009 Feb 4. 28(3):274-85.

    Insert

    Programmed Cell Death Protein 4 (PDCD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    I36V- does not alter function

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

20. wt Opa1

    Plasmid

    #204427

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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