We narrowed to 15,727 results for: NOL

1. AA299

   Plasmid

   #216029

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Slug]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA296

   Plasmid

   #216028

   Purpose

   Fragmid fragment: (Cas protein) NNNNCC PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Nme2Cas9_v1.1 [Nme2]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA180

   Plasmid

   #216023

   Purpose

   Fragmid fragment: (Cas protein) NGRR(T) PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sa]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AA301

   Plasmid

   #216030

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sauri]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AA027

   Plasmid

   #216007

   Purpose

   Fragmid fragment: (Cas protein) nearly PAM-less

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpRY_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AA026

   Plasmid

   #216006

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-SpG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LentiGuide-Puro-GFPg1 (BB09)

   Plasmid

   #139461

   Purpose

   Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   GFP-targeting sgRNA inserted; resistance gene: puroR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS_COV2_ORF3b_HA_BASU

   Plasmid

   #153992

   Purpose

   Express Orf3b gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf3b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. STARR-seq luciferase validation vector_SCP1_empty

   Plasmid

   #99299

   Purpose

   Luciferase validation vector; empty vector without insert to determine baseline

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Luciferase; Starr-seq luciferase validation vector

   Expression

   Mammalian

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCpf1-BE-YE

    Plasmid

    #107686

    Purpose

    Expresses dLbCpf1-BE-YE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YE
    

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDR348 FANCF

    Plasmid

    #47510

    Purpose

    human gRNA expression vector targeting FANCF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-FANCF (FANCF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid

    #112031

    Purpose

    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    

    Tags

    Split mCherry

    Expression

    Bacterial

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-(pos36)GFP-9xGGS-Cre-6xHis

    Plasmid

    #62938

    Purpose

    Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (ps36)GFP-9xGGS-Cre
    

    Tags

    (pos36)GFP and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ctcf-Halo-mAID donor

    Plasmid

    #113103

    Purpose

    Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC119

    Plasmid

    #118884

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and mGFP, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Tec-SH2

    Plasmid

    #46522

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Tec (TEC Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 236-351

    Promoter

    Tac

    Available Since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G03 BATF3

    Plasmid

    #67050

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BATF3 (BATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Expression

    Plant

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191155

    Purpose

    Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:DNAJB1.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX Stat6-SH2

    Plasmid

    #46517

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat6 (STAT6 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 510-654

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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