We narrowed to 32,277 results for: uros

1. Ai62(TITL-tdT) Flp-in replacement vector

   Plasmid

   #61576

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   chromatin insulator flanked TRE-LSL-tdTomato
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rasgrf2-2A-dCre targeting vector

   Plasmid

   #61573

   Purpose

   Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Rasgrf2-2A-dCre (Rasgrf2 Mouse, P1 bacteriophage)
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   March 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

   Plasmid

   #209196

   Purpose

   Mutagenesis of Cnr1

   Depositor

   Larry Zweifel

   Article

   Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

   Insert

   Cnr1 (Cnr1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Promoter

   CMV

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV Marina-T2A-nls-mCherry

   Plasmid

   #74216

   Purpose

   genetically-encoded fluorescent voltage indicator

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   fluorescent protein voltage sensor
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   CMV

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMMS22L-siR3

   Plasmid

   #221013

   Purpose

   mammalian expression vector of myc-Flag tagged MMS22L siRNA resistant

   Depositor

   Daniel Durocher

   Article

   O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

   Insert

   MMS22L (MMS22L Human)
   

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   silent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…

   Promoter

   CMV

   Available Since

   July 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pN1 Lck-GCaMP5G

   Plasmid

   #34924

   Purpose

   Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

   Depositor

   Baljit Khakh

   Article

   Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

   Insert

   Lck-GCaMP5G
   

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Promoter

   CMV

   Available Since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2plus-TDP-43 M337V

   Plasmid

   #162609

   Purpose

   Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   M337V

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-FLEX-SomaGCaMP7

   Plasmid

   #182942

   Purpose

   Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Insert

   Soma-GCaMP7
   

   Use

   AAV

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pBG-mCRE98-NLS-EGFP-WPRE

   Plasmid

   #239977

   Purpose

   adeno-associated virus (AAV) encoding enhanced green fluorescent protein (eGFP) under motor neuron-selective control of minimal beta globin promoter (pBG) and CRE98

   Depositor

   Sinisa Hrvatin

   Article

   Nagy et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   EGFP (eGFP Synthetic)
   

   Use

   AAV

   Promoter

   pBG + mCRE98

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

    Plasmid

    #52504

    Purpose

    mammalian expression vector of BRCA1 wildtype siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: AGTATAATC

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pORANGE GFP-CACNA1A KI

    Plasmid

    #131480

    Purpose

    Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-DIO-PPO-Venus

    Plasmid

    #139505

    Purpose

    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Has Service

    AAV8 and AAV9

    Insert

    parapinopsin
    

    Use

    AAV

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CIRTS-FTO-control

    Plasmid

    #216859

    Purpose

    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-FTO-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Promoter

    Syn1, U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn Marina-T2A-nls-mCherry

    Plasmid

    #85843

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    GEVI Marina
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    hSyn1

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL-CRISPR.EFS.PAC

    Plasmid

    #57828

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. elavl3:SomaGCaMP7f

    Plasmid

    #158760

    Purpose

    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP7
    

    Use

    Synthetic Biology

    Promoter

    HuC promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CIRTS-YTHDF2-control

    Plasmid

    #216857

    Purpose

    CIRTS RNA targeting system for targeted knockdown of m6A-containing transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-YTHDF2-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn1, U6

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATM gRNA (BRDN0001149518)

    Plasmid

    #77529

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATM

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ATM (ATM Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-eGFP-SHLD3

    Plasmid

    #114125

    Purpose

    Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAAV-EF1a-FLEX-SomaGCaMP7

    Plasmid

    #182941

    Purpose

    Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    Soma-GCaMP7
    

    Use

    AAV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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