We narrowed to 73,426 results for: LAS

1. pNSU299_SEO1

   Plasmid

   #166076

   Purpose

   Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   SEO1 (SEO1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Pf-GMPS

   Plasmid

   #36899

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Pf-GMPS (PF10_0123 Plasmodium falciparum)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pUC19-link-PAGFP-FLAG:Kan

   Plasmid

   #211834

   Purpose

   Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   PAGFP-FLAG-Kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV3XFLAGm43CT

   Plasmid

   #17664

   Depositor

   Cecilia Lo

   Insert

   Cx43 CT (Gja1 Mouse)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Cytoplasmic tail: aa 236-382

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4134897

   Plasmid

   #151948

   Purpose

   Mammalian expression plasmid for Human coronavirus HKU1 nucleocapsid

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   Human coronavirus HKU1 nucleocapsid phosphoprotein (N Human coronavirus HKU1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pCB52

   Plasmid

   #69747

   Purpose

   Retroviral expression of Cyclin B1

   Depositor

   Iain Cheeseman

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Retroviral

   Tags

   eGFP-TEV-S

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

7. p406GALS

   Plasmid

   #17422

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   p406GALS (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALS promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

   Plasmid

   #186047

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8f
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

   Plasmid

   #186048

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8m
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKM510

    Plasmid

    #134227

    Purpose

    Retroviral plasmid expressing mCherry - HJURP

    Depositor

    Iain Cheeseman

    Insert

    mCherry-HJURP-LacI 1-304 (HJURP Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid

    #195487

    Purpose

    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C61T, C262V

    Promoter

    T7

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mT2A-td-sfGFP-FNF

    Plasmid

    #182339

    Purpose

    Recombineering cassette plasmid for T2A-td-sfGFP-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-td-sfGFP-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Z-MATR3-4

    Plasmid

    #166558

    Purpose

    scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST26-his-hSFRP1

    Plasmid

    #189539

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPK719

    Plasmid

    #38149

    Depositor

    Patricia Kuwabara

    Insert

    cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)
    

    Use

    Recombineering

    Tags

    Expression

    Worm

    Mutation

    Promoter

    unc-119

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV Tor1a-Myc

    Plasmid

    #175134

    Purpose

    Lentiviral expression of Myc-tagged mouse Tor1a

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS ZfHoxb1a

    Plasmid

    #27519

    Depositor

    Victoria Prince

    Insert

    Hoxb1a (hoxb1a Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid

    #160587

    Purpose

    Nickase D10A Cas9 for Nt and Ct fusion.

    Depositor

    Diego Orzaez

    Insert

    nickase D10A Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

    Plasmid

    #102771

    Purpose

    "Activate neurons with 2p stimulation"

    Depositor

    Karel Svoboda

    Insert

    C1V1::FusionRed::Kv2.1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1 traficking domain

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SPIN3A

    Plasmid

    #74656

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPIN3A (SPIN3 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only