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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 8521 - 8540 of 18351 results

  1. pcDNA3-RAB-AKARev(T/A)

    Plasmid
    #118479
    Purpose
    Negative-control mutant for RAB-AKARev biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-AKARev(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118479-plasmid-map-sequence-id-230370

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  3. ACTB-(A)3x

    Plasmid
    #112057
    Purpose
    ACTB mRNA tagged with 3 copies of Riboglow (variant A)
    Depositor
    Amy Palmer
    Publication
    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB (ACTB Human)
    Tags
    Riboglow RNA tag
    Expression
    Mammalian
    Mutation
    3 copies of Riboglow tag (variant A) after stop c…
    Promoter
    CMV
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112057-plasmid-map-sequence-id-214332

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  4. ACTB-(A)2x

    Plasmid
    #112056
    Purpose
    ACTB mRNA tagged with 2 copies of Riboglow (variant A)
    Depositor
    Amy Palmer
    Publication
    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB (ACTB Human)
    Tags
    Riboglow RNA tag
    Expression
    Mammalian
    Mutation
    2 copies of Riboglow tag (variant A) after stop c…
    Promoter
    CMV
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112056-plasmid-map-sequence-id-214330

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  7. pKS7107

    Plasmid
    #89051
    Purpose
    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Nm crRNA
    Nm tracrRNA
    hNmCas9
    Use
    CRISPR
    Tags
    HA tag, NLS, and SV40 NLS
    Promoter
    EF1a and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89051-plasmid-map-sequence-id-176334

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  8. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75177
    Purpose
    Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75177-plasmid-map-sequence-id-145999

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  9. pMO14

    Plasmid
    #235736
    Purpose
    Expression of human VPS34 complex II in mammalian cells
    Depositor
    Roger Williams
    Publication
    Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    UVRAG (UVRAG Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    Tags
    2xStrep-tagII-2xTEV-ZZ
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235736-plasmid-map-sequence-id-472856

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  11. pLV-SFFV-KTN1-WPRE-UbC-Emerald

    Plasmid
    #225941
    Purpose
    Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    KTN1 (KTN1 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225941-plasmid-map-sequence-id-446792

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  12. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid
    #229539
    Purpose
    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
    Depositor
    Bernhard Lechtenberg
    Publication
    Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025
    Inserts
    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    Tags
    6xHis-3C
    Expression
    Bacterial
    Mutation
    Changed Gly 76 to Val and changed Gly 76 to Val
    Promoter
    T7
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    229539-plasmid-map-sequence-id-456666

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  14. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid
    #194470
    Purpose
    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Publication
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Mutation
    Aspartic Acid 242 (in Dennis et al.) changed to A…
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    194470-plasmid-map-sequence-id-384877

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  15. pEGFP_D2A_tdTomato

    Plasmid
    #184045
    Purpose
    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)
    Depositor
    Angela Pannier
    Publication
    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Enhanced Green Fluorescent Protein
    tdTomato
    Dual 2A Peptide Sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184045-plasmid-map-sequence-id-363159

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  17. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

    Plasmid
    #188991
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Publication
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLD
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    F163L, K249R, G328S, G381V, G429D, D480G
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188991-plasmid-map-sequence-id-371644

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  20. pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVI

    Plasmid
    #181704
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Publication
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181704-plasmid-map-sequence-id-354499

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Showing: 8521 - 8540 of 18351 results