We narrowed to 17,096 results for: Por

1. pS238D1::sptse5-CT_phoA-lacZalpha

   Plasmid

   #192961

   Purpose

   Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT_phoA-lacZ_
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317) fused to phoA-lacZal…

   Promoter

   Available Since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKTop::tse5-CT (1169-1229)

   Plasmid

   #192953

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1229) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1229)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1229)

   Promoter

   Available Since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKTop::sptse5-CT (1169-1281)

   Plasmid

   #192950

   Purpose

   For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1281)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Promoter

   Available Since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKTop::sptse5-CT (1169-1269)

   Plasmid

   #192949

   Purpose

   For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1269)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1269)

   Promoter

   Available Since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKTop::sptse5-CT (1169-1300)

   Plasmid

   #192951

   Purpose

   For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1300)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for sptse5 (1169-1300)

   Promoter

   Available Since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9-EF-5Flnc

   Plasmid

   #174870

   Purpose

   CRISPR vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Flnc (Flnc Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2326

   Plasmid

   #169628

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTS2321

   Plasmid

   #169626

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. fru-T2A-QF2 HDR plasmid

   Plasmid

   #141099

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

   Depositor

   Leslie Vosshall

   Article

   Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

   Insert

   fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSN554

    Plasmid

    #177361

    Purpose

    Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

    Depositor

    Shinsuke Niwa

    Article

    Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    KIF1A (KIF1A Human)
    

    Use

    Tags

    Leucine zipper, StrepII tag, and mScarlet

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid

    #184636

    Purpose

    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Gat1 gene with Arginine 69 changed to Lysine, to …

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-mitoX4-FKBP-HA

    Plasmid

    #185658

    Purpose

    Mitochondrial targetted (Cox8) FKBP protein with C-terminal HA epitope

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    mito-FKBP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTS2329

    Plasmid

    #169629

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS2322

    Plasmid

    #169627

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTS2320

    Plasmid

    #169625

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS2312

    Plasmid

    #169622

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS2317

    Plasmid

    #169624

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT22iU66CriT(#387)

    Plasmid

    #184061

    Purpose

    cyclofen-inducible CRE activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHcRed-NPMMLF1nes-C1

    Plasmid

    #131849

    Purpose

    Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a control deletion contruct of the cytop…

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQCXIP-SHMY-A1Pi

    Plasmid

    #182648

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    A1Pi (SERPINA1 Human)
    

    Use

    Retroviral

    Tags

    His6-tag, N-terminal ER targeting sequence of hem…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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