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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,185 results for: SHA

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Showing: 8561 - 8580 of 12185 results

  1. pPICZc-ScAct1*-thymosinB-8His

    Plasmid
    #111148
    Purpose
    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    Act1 (ACT1 Budding Yeast)
    Use
    Pichia pastoris integration plasmid
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Promoter
    AOX1
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111148-plasmid-map-sequence-id-214055

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  3. qTAG-C-moxGFP-Puro-LAMP1

    Plasmid
    #207788
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207788-plasmid-map-sequence-id-416383

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  5. qTAG-C-mStayGold-EFS-Blast-ARL13B

    Plasmid
    #227278
    Purpose
    Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227278-plasmid-map-sequence-id-451732

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  6. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid
    #141184
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has Service
    Cloning Grade DNA
    Insert
    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    Tags
    Signal peptide from influenza HA and Superfolder …
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141184-plasmid-map-sequence-id-306284

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  9. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid
    #145145
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Tags
    8his tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145145-plasmid-map-sequence-id-324821

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  10. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid
    #207792
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207792-plasmid-map-sequence-id-413795

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  18. pCEP4-myc-ACE2

    Plasmid
    #141185
    Purpose
    Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141185-plasmid-map-sequence-id-274835

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  19. qTAG-C-Solo-mStayGold-COX8A

    Plasmid
    #227309
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227309-plasmid-map-sequence-id-451688

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  20. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid
    #141183
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141183-plasmid-map-sequence-id-288058

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Showing: 8561 - 8580 of 12185 results