167,910 results

1. AICSDP-55:DMD-mEGFP

   Plasmid

   #159744

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_SPP1_WT_V5

   Plasmid

   #82981

   Purpose

   Gateway Donor vector containing SPP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SPP1 (SPP1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS mouse IRS-2

   Plasmid

   #11372

   Depositor

   Ronald Kahn

   Article

   Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

   Insert

   IRS-2 (Irs2 Mouse)
   

   Expression

   Bacterial

   Available Since

   March 1, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCLX-pTF-R1-DEST-R2-EBR65

   Plasmid

   #45952

   Depositor

   Patrick Salmon

   Article

   Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-BFP-CAAX

   Plasmid

   #184459

   Purpose

   Plasma membrane-targeted EBFP2 expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   EBFP2-CAAX
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-dCLK

   Plasmid

   #33153

   Depositor

   Michael Rosbash

   Article

   McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

   Insert

   CLK (Clk Fly)
   

   Use

   Retroviral

   Tags

   6xHIS and V5

   Expression

   Insect

   Promoter

   Actin 5C

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pNB23

   Plasmid

   #103058

   Purpose

   medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

   Depositor

   Dirk Bumann

   Article

   Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

   Insert

   GFP-OVA
   

   Expression

   Bacterial

   Promoter

   hydrogen peroxide-inducible promoter katGp

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX301-KANK1

   Plasmid

   #121985

   Purpose

   overexpress of KANK1

   Depositor

   GuangJun Zhang

   Article

   Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

   Insert

   KANK1 (KANK1 Human)
   

   Use

   Lentiviral

   Promoter

   CMV promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKB01_mOrange2

   Plasmid

   #69102

   Purpose

   Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mOrange2
   

   Use

   Synthetic Biology

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-FRISZ

    Plasmid

    #176887

    Purpose

    Far-red fluorescent zinc indicator FRISZ in pDisplay

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    FRISZ
    

    Tags

    pDisplay backbone elements for export and membran…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMP4639

    Plasmid

    #107771

    Purpose

    Expression of EYFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EYFP in pME6010
    

    Tags

    pLacZ-EYFP

    Expression

    Bacterial

    Promoter

    pLacZ

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-TALO8

    Plasmid

    #83409

    Purpose

    TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    TRP-ARS-8xLacO
    

    Expression

    Yeast

    Mutation

    Please see depositor comments below

    Promoter

    TRP1

    Available Since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMVP-Cas9FL-P2A-turboGFP

    Plasmid

    #80941

    Purpose

    Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9FL
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. ptwB

    Plasmid

    #48735

    Purpose

    Tandem dual tomato plasmid

    Depositor

    John Schiller, Roger Tsien

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    Tandem dual tomato
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5VBD (USP09X)

    Plasmid

    #101260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP09X (USP9X Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pVITRO-HPV38 L1L2

    Plasmid

    #52594

    Purpose

    Expresses HPV38 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV38 L1
    HPV38 L2
    

    Expression

    Mammalian

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNES-EGFP-C1-flex-PABD-Spo20

    Plasmid

    #220081

    Purpose

    Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Tags

    X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

    Expression

    Mammalian

    Mutation

    amino acids 51-91

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV V5-LAP2beta

    Plasmid

    #175112

    Purpose

    Lentiviral expression of V5-tagged mouse Tmpo

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmpo (Tmpo Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. DL034:cFos::HaloTag-iPAK4

    Plasmid

    #177884

    Purpose

    HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    cFos

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pT7-7 asyn S129A

    Plasmid

    #36048

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    S129A

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBS MCK CRE

    Plasmid

    #12529

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Transgenic construct

    Mutation

    Cre is driven by the Muscle Creatine Kinase promo…

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDONR221_SLC2A12

    Plasmid

    #132067

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A12 (SLC2A12 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. PRRX2-pSG5

    Plasmid

    #21009

    Depositor

    Corey Largman

    Article

    Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

    Insert

    PRRX2 (Prrx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

    Plasmid

    #171963

    Purpose

    Donor plasmid for CLTX T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a-tm CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLuc-3'UTR

    Plasmid

    #42094

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 3UTR (ZEB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

    Plasmid

    #175177

    Purpose

    Cre-dependent GCaMP6s and mBeRFP expression

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-mBeRFP

    Promoter

    CAG

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLV sgRNA-Notch1 #1 GFP

    Plasmid

    #106950

    Purpose

    Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    gRNA targeting Notch1 (Notch1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pC1-IMS-oROS-HT

    Plasmid

    #216419

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAL1

    Plasmid

    #197285

    Purpose

    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMpGWB123

    Plasmid

    #68577

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Triple repeats of Citrine (3xCitrine)

    Expression

    Plant

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET SUMO NL-FKBP-Cas9

    Plasmid

    #162730

    Purpose

    Bacterial expression, protein expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GST-Rab8a-QL

    Plasmid

    #86079

    Purpose

    A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPOTv7-g418-3mNG

    Plasmid

    #179832

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean, Keith Gull

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3x mNG
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA5/FRT/TO HIS HSPA6

    Plasmid

    #19540

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. gRNA-HEK3-Puro

    Plasmid

    #136282

    Purpose

    Expresses a guide RNA targeting HEK3 site

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    sgRNA-HEK3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDONR223_RIMS2_WT

    Plasmid

    #81803

    Purpose

    Gateway Donor vector containing RIMS2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RIMS2 (RIMS2 Human)
    

    Use

    Gateway entry vector

    Mutation

    S86F; Q295R

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBW-m4133611

    Plasmid

    #152146

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. GST-Rab8a-TN

    Plasmid

    #86080

    Purpose

    A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge