We narrowed to 1,196 results for: MS2;

1. Fluorescent reporter for MOBE3-4

   Plasmid

   #219946

   Purpose

   Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   EGFP(A111V/L202S)

   Promoter

   CMV, U6

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ131A2.0

   Plasmid

   #99884

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCH6 - pBait-ChiX

   Plasmid

   #174662

   Purpose

   arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Insert

   ChiX
   

   Use

   Tags

   1xMS2 hairpin

   Expression

   Bacterial

   Mutation

   Promoter

   pBad

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ132A2.0

   Plasmid

   #99887

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ133A2.0

   Plasmid

   #99891

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ133C2.0

   Plasmid

   #99893

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ131C2.0

   Plasmid

   #99886

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU6

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ132C2.0

   Plasmid

   #99889

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ141-ZmUbi-RZ-Aac.4

    Plasmid

    #158406

    Purpose

    Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ132D2.0

    Plasmid

    #99890

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ141-ZmUbi-RZ-Aa3.8.5

    Plasmid

    #158407

    Purpose

    Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ133D2.0

    Plasmid

    #99894

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ131D2.0

    Plasmid

    #100044

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MCP-mCherry-TIS11B

    Plasmid

    #207670

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    TIS11B (ZFP36L1 Human)
    

    Use

    Tags

    MCP-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. THI ONLY pALD4 T-

    Plasmid

    #161497

    Purpose

    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

    Plasmid

    #79380

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

    Plasmid

    #79379

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

    Plasmid

    #79382

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge