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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230990

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-CTR1-shRNA

Plasmid

#53180

Purpose

Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

Plasmid

#110055

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

Plasmid

#221329

Purpose

Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)

Use

Retroviral

Mutation

G12V

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE

Plasmid

#192202

Purpose

Retro-HER2CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR-PRODH2 (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

Plasmid

#22132

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

Plasmid

#46277

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28a-GFP-Patronin CC 868–1457

Plasmid

#59053

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

His, Strep, TEV, and sfGFP

Expression

Bacterial

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

Plasmid

#203439

Purpose

Mammalian expression of RUNX4

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Tags

3 x FLAG

Expression

Mammalian

Mutation

R122C

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA

Plasmid

#62931

Purpose

pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

Lentiviral

Promoter

1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

Plasmid

#105182

Purpose

Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-ECFP (Cerulean) sponge control

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI control for Target-AID (pRZ1047)

Plasmid

#131299

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
pET-21a-GFP-Patronin CC 1288–1457

Plasmid

#59055

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-CMV-intron-GFP-pA

Plasmid

#192493

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-CMV-intron-MCS-pA

Plasmid

#192496

Purpose

To express CasRX gRNA and contains MCS to insert coding region of interest

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-Positron2-p10

Plasmid

#239082

Purpose

Gal4 activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2

Expression

Insect

Mutation

R78K N81D D92N W178F

Promoter

hsp70

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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