167,808 results

1. repressilator (pZS1-lTlrLLtCL)

   Plasmid

   #26489

   Depositor

   Michael Elowitz

   Article

   Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

   Insert

   LacI, tetR
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWPI-LUC7L2-3xFLAG

   Plasmid

   #201641

   Purpose

   Over-expression of 3xFLAG-tagged human LUC7L2

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L2 (LUC7L2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-6xHis-MBP-TEV site-TadA8.20

   Plasmid

   #194702

   Purpose

   Expresses N-terminal MBP fused TadA8.20 in bacteria

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   TadA8.20
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   T7 promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 luciferase

   Plasmid

   #26612

   Depositor

   Jie Chen

   Article

   Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

   Insert

   firefly luciferase
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

5. Opie2-PuroR

   Plasmid

   #131613

   Purpose

   Expresses PuroR and dsRed.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR
   dsRed
   

   Expression

   Insect

   Promoter

   Opie2 and hr5Ie1

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPUR-mU6-sgRNA-2XPP7

   Plasmid

   #194504

   Purpose

   Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-GCK

   Plasmid

   #23750

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   GCK (GCK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK1334

   Plasmid

   #127965

   Purpose

   sgRNA expression vector compatible with CROP-Seq and imaging screens

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HaloTag-beta2-adrenergic receptor

   Plasmid

   #66994

   Purpose

   Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-beta2-adrenergic receptor (ADRB2 Human)
   

   Tags

   Human Growth hormone Signal Sequence-HaloTag

   Expression

   Mammalian

   Mutation

   The signal sequence from human growth hormone was…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E1D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKRaION1-N1

    Plasmid

    #177801

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. miCBE

    Plasmid

    #205413

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
    

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-link-ymNeongreen-URA3

    Plasmid

    #168059

    Purpose

    Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Tags

    ymNeonGreen

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-fDIO-Cre (AAV5)

    Viral Prep

    #121675-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

16. mVenus-pBAD

    Plasmid

    #54845

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Tags

    6xHis and mVenus

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. EF06R

    Plasmid

    #42940

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    Human L1 element (L1RE1 Human)
    

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Promoter

    CMV MIE, L1 5'UTR

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLB1080

    Plasmid

    #37826

    Depositor

    Ann Hubbard

    Article

    Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

    Insert

    ATP7B (ATP7B Human)
    

    Tags

    mEGFP

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. ClCry4 PHR

    Plasmid

    #160552

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 HR
    

    Tags

    Hexa his

    Expression

    Insect

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-ChrimsonR-mCherry

    Plasmid

    #92207

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    ChimsonR-mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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