227,697 results

1. pmScarlet-i_H2A_C1

   Plasmid

   #85053

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   H2A (H2AC16 Human)
   

   Use

   Tags

   mScarlet-i

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB061i3

   Plasmid

   #79230

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP.N1-HDAC6.DC (catalytic deficient)

   Plasmid

   #36189

   Depositor

   Tso-Pang Yao

   Article

   Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

   Insert

   HDAC6.DC (HDAC6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H216/611A

   Promoter

   CMV

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLminP_Luc2P_RE57

   Plasmid

   #90402

   Purpose

   Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   GR

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSAM_AraC

   Plasmid

   #91569

   Purpose

   Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

   Depositor

   Harry Mobley

   Article

   Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

   Insert

   AraC/PBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRK892

   Plasmid

   #71803

   Purpose

   Expression of mutated L. gasseri gusA from strong Ppgm promoter

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

   Insert

   gusA3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D573A

   Promoter

   Ppgm

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6-CENP-A-mCherry

   Plasmid

   #89767

   Purpose

   Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CENP-A (CENPA Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPI-N1ICD

   Plasmid

   #185525

   Purpose

   Constitutive overexpression of human NOTCH1 intracellular domain

   Depositor

   Eric Shusta, Sean Palecek

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-HA-USP48

   Plasmid

   #22585

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP48 (USP48 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCVpuro-Cre

    Plasmid

    #34564

    Purpose

    Retroviral expression of Cre

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. shYAP1 # 2

    Plasmid

    #42541

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-MCS1-P2A-MCS2 (Hygro)

    Plasmid

    #80922

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFPC1-muscle Amphiphysin 2

    Plasmid

    #22213

    Depositor

    Pietro De Camilli

    Article

    Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

    Insert

    Amphiphysin II (BIN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cyto-roGFP

    Plasmid

    #49435

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    roGFP2
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. LcV2-Hygro

    Plasmid

    #91977

    Purpose

    LentiCRISPR v2 with Hygro cloned in place of Puro

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. shYAP1 # 1

    Plasmid

    #42540

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His-FGE

    Plasmid

    #75147

    Purpose

    Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

    Depositor

    Richard Fishel

    Article

    Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

    Insert

    FGE
    

    Use

    Tags

    Histidine tag and Thrombin

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    T7

    Available since

    July 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pRSET FlipSuc-90u delta1

    Plasmid

    #14475

    Depositor

    Wolf Frommer

    Article

    Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

    Insert

    FlipThuE W283A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    N192D, W283A, 13 amino acids deleted from linkers

    Promoter

    T7

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge