We narrowed to 9,668 results for: BLI

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pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

Plasmid

#225699

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV1E

Plasmid

#224439

Purpose

Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDTMSK insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Purpose

Gateway entry vector containing MEKDD.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Mutation

S218D S222D

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV1C

Plasmid

#224438

Purpose

Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDLSTP insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225702

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV2E

Plasmid

#224441

Purpose

Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDQGYQ insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3D

Plasmid

#224445

Purpose

Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYREL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3E

Plasmid

#224446

Purpose

Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHGVL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4B

Plasmid

#224449

Purpose

Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYTSV insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4D

Plasmid

#224451

Purpose

Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHGVL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4C

Plasmid

#224450

Purpose

Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYTSM insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV3C

Plasmid

#224444

Purpose

Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYSSV insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV3B

Plasmid

#224443

Purpose

Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYSGL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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AID-C12*-BE4max

Plasmid

#216729

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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SspB-HaloTag-DHPH-ITSN1

Plasmid

#176113

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-PURO-LYSET-gRNA1

Plasmid

#210489

Purpose

CRISPR pooled KO of human LYSET

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-gRNA1 (LYSET Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-PURO-LYSET-gRNA2

Plasmid

#210490

Purpose

CRISPR pooled KO of human LYSET

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-gRNA2 (LYSET Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-ITSN1

Plasmid

#176117

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VAMP2 R125TAG

Plasmid

#69876

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2-GFP (Vamp2 Rat)

Use

Synthetic Biology

Tags

GFP

Expression

Mammalian

Mutation

Amber stop codon in the linker between VAMP2 and …

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

Plasmid

#194190

Purpose

Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

5 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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