We narrowed to 10,125 results for: Uty

1. pCXLE-dCas9VPPH-T2A-GFP-shP53

   Plasmid

   #102897

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPPH-T2A-GFP-shP53
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Tn7-157

   Plasmid

   #118571

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mClover3
   

   Use

   Minitn7 delivery vector

   Expression

   Bacterial

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FnoCas12a-2C-NLS in pCSDest

   Plasmid

   #126639

   Purpose

   Expresses FnoCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Promoter

   CMV IE94 promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest27-mBIN1

   Plasmid

   #61086

   Purpose

   Express N-terminal GST tagged-mouse BIN1 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   smallest constitutive BIN1excluding exon 7, 11, 13-17
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. TRE-mRuby3

   Plasmid

   #214923

   Purpose

   expression vector control - contitutive expression of mRuby33 alone

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   mRuby3
   

   Use

   Lentiviral

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

   Plasmid

   #178585

   Purpose

   Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   DLX2 (DLX2 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-cFos-mCh

   Plasmid

   #193066

   Purpose

   constitutive expression of mouse cFos fused to mCherry in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   cfos-mCh
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBeast-pT7-soxA

   Plasmid

   #190057

   Purpose

   Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   SoxA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PhyB-mCherry-CDC10

   Plasmid

   #51568

   Purpose

   Constitutively express bud neck localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   CDC10 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid

    #49520

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38M-P750-luxAB-DAS+4
    

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK389.AAV

    Plasmid

    #177327

    Purpose

    aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

    Depositor

    James Carothers

    Article

    Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

    Inserts

    SoxS
    dCas9
    

    Use

    CRISPR

    Tags

    MCP (MS2 Coat Protein)

    Mutation

    R93A,S101A

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDule-IBBN (G2)

    Plasmid

    #85500

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-GAR

    Plasmid

    #34697

    Depositor

    Steven Clarke

    Article

    Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-145 of human fibrillarin with K2E and …

    Promoter

    tac

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pMRE-Tn5-146

    Plasmid

    #118530

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV7.1_3xFLAG-MOB1A

    Plasmid

    #172988

    Purpose

    constitutive expression of FLAG-tagged MOB1A

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-MOB1A (MOB1A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PhyB-mCherry-Spc72

    Plasmid

    #51582

    Purpose

    Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    Spc72 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid

    #165907

    Purpose

    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLIK-Venus-TmiR-Luc

    Plasmid

    #25739

    Purpose

    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pMRE-Tn5-151

    Plasmid

    #118533

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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