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We narrowed to 10,211 results for: FIE

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Showing: 8681 - 8700 of 10211 results

  2. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid
    #122064
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1a（1.1kb short version)
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122064-plasmid-map-sequence-id-242270

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  3. pYeDP60-hATP13A2-E343A

    Plasmid
    #171378
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Article
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    E343A
    Promoter
    URA3
    Available Since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171378-plasmid-map-sequence-id-342930

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  5. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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  6. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid
    #102421
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Article
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Modified at the Dharmacon SMARTpool siRNA #2 targ…
    Promoter
    TetO-CMV
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102421-plasmid-map-sequence-id-198790

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  7. pTripz NEO DEST

    Plasmid
    #209072
    Purpose
    Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector to be used…
    Tags
    3X FLAG
    Promoter
    CMV
    Available Since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209072-plasmid-map-sequence-id-412301

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  8. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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  9. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

    Plasmid
    #84483
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available Since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84483-plasmid-map-sequence-id-177044

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  12. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128587
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α (1.1 kb short version)
    Available Since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128587-plasmid-map-sequence-id-257676

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  13. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid
    #223167
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Article
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223167-plasmid-map-sequence-id-442870

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  15. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

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  16. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid
    #171995
    Purpose
    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171995-plasmid-map-sequence-id-341857

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  17. Cer(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #55708
    Purpose
    An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
    Tags
    Cer(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-5 was amplified via PCR, which added an N-t…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55708-plasmid-map-sequence-id-114838

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  18. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid
    #197268
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-v2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197268-plasmid-map-sequence-id-389661

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Showing: 8681 - 8700 of 10211 results