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pGV3340

Plasmid

#170278

Purpose

Expression of mutant HIV-2 RT p51 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p51 (mutant)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-HLA-cmyc-optiT1R3 a21-852

Plasmid

#113948

Purpose

mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 21-852

Promoter

CMV

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ACBEmax

Plasmid

#139106

Purpose

Mammalian ACBEmax expression vector (pSI-632)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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NSK026 CMV-TO-p53R248W (FLP-IN)

Plasmid

#191168

Purpose

Expression of human p53 mutant R248W in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

p53(R248W) (TP53 Human)

Expression

Mammalian

Mutation

R248W

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-D131Y

Plasmid

#113424

Purpose

Expresses hA3A-BE3-D131Y in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-D131Y (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_D131Y

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
pCMV-hA3C-BE3

Plasmid

#113412

Purpose

Expresses hA3C-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3C-BE3 (APOBEC3C S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMJA219= pcDNA3.1V5-completeTRABV-CMVp

Plasmid

#116878

Purpose

mammalian expression vector containing TCR sequences

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)

Expression

Mammalian

Promoter

CMV Promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDRIVE-CAG-hFX-HA

Plasmid

#14994

Depositor

Michael Ristow

Article

Schulz et al Aging (Albany NY). 2010 Nov;2(11):843-53

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001146145)

Plasmid

#80198

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A8

Plasmid

#132177

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A8 (SLC39A8 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Circular 200,100 with interspersed loops (FANCC)

Plasmid

#180192

Purpose

AAV vector carrying a guide RNA targeting the human FANCC mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (CTNNB1)

Plasmid

#180193

Purpose

AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MAVS-ts3

Plasmid

#174276

Purpose

MAVS knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MAVS shRNA (MAVS Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh1.0

Plasmid

#106072

Purpose

express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 1.0

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-NL-GW

Plasmid

#113446

Purpose

NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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ABE8.17-d

Plasmid

#136299

Purpose

expresses ABE8.17-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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NKP90 RFP (787)

Plasmid

#62001

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

NKP90 (TMEM126A Human)

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pYPQ265F2

Plasmid

#164722

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)NG-UGI

Use

CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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