We narrowed to 45,425 results for: Ina

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pCMV sMTase (eGFP)

Plasmid

#89930

Purpose

Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC-IRES-MN

Tags

Flag and HA

Expression

Mammalian

Mutation

deactivated Cas9

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-PKI nls

Plasmid

#118301

Purpose

Expression of PKIa

Depositor

Philip Stork

Article

Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

Insert

PKI nls (aa 2-36)

Tags

Flag and GFP

Expression

Mammalian

Mutation

inserting 2xnls

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC840 - pAAV 5xATF6 secNLuc

Plasmid

#82497

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

5x(ATF6 binding site) c-Fos minimal promoter

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-V5

Plasmid

#180413

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Fyn

Plasmid

#16033

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

Dominant negative: K299M

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA SF-HERC2 F6

Plasmid

#55789

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pAM-AAV-mSncg-0.27kb-EGFP

Plasmid

#153185

Purpose

Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCPΔCR

Plasmid

#83239

Purpose

The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCpdCr

Tags

roGFP2 fusion with Tsa2dCpdCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLHCX-FLAG-mPKM1

Plasmid

#44240

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M1 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
GFP-JNK2

Plasmid

#86831

Purpose

mammalian expression of GFP-JNK2

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK2 (MAPK9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M337V

Plasmid

#98674

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

M337V

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_Q331K

Plasmid

#98673

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

Q331K

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

Plasmid

#184951

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mCherry

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-LATS2-KR

Plasmid

#33100

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

lats2 (Lats2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

K655R, Kinase Dead

Promoter

CMV

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE-UBC-hAAT-IRES-ZsGreen-W

Plasmid

#126702

Purpose

lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Insert

Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)

Use

Lentiviral

Promoter

UBC

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
MSP6-bio

Plasmid

#47732

Purpose

Expresses enzymatically monobiotinylated full-length MSP6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MSP6

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_SEPT9 i1

Plasmid

#71609

Purpose

mammalian expression of human SEPT9 i1 with a GFP fusion.

Depositor

Cristina Montagna, Elias Spiliotis

Article

Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

Insert

Septin 9 (SEPTIN9 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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