Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 22,848 results for: Tes

Showing: 8761 - 8780 of 22848 results

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pAG415Gal_Gcn4 S/ArotoL

Plasmid

#232979

Purpose

Galactose iduced expression of Gcn4 S/ArotoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED WHA

Plasmid

#232995

Purpose

Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAHA

Plasmid

#232997

Purpose

Galactose iduced expression of Gcn4 9acidAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidA

Plasmid

#232967

Purpose

Galactose iduced expression of Gcn4 9acidA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAGFP

Plasmid

#233009

Purpose

Galactose iduced expression of Gcn4 9acidAGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+GFP

Plasmid

#233002

Purpose

Galactose iduced expression of Gcn4 Aro3+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED

Plasmid

#232957

Purpose

Galactose iduced expression of Gcn4 ILVtoED in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED WGFP

Plasmid

#233007

Purpose

Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-AncHolozoan Sox

Plasmid

#231528

Purpose

Expresses reconstructed Ancestral Holozoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Holozoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-AncMetazoan Sox

Plasmid

#231527

Purpose

Expresses reconstructed Ancestral Metazoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Metazoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-AncSoxCEF

Plasmid

#231526

Purpose

Expresses reconstructed Ancestral SoxCEF in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral SoxCEF HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-AncSoxB

Plasmid

#231524

Purpose

Expresses reconstructed Ancestral SoxB in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral SoxB HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Pchi-HMG

Plasmid

#231540

Purpose

Expresses the HMG of Pchi Sox with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox HMG

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Salhel-HMG

Plasmid

#231539

Purpose

Expresses the HMG of Salhel Sox-I with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I HMG

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Myflu-POU

Plasmid

#231542

Purpose

Expresses the DNA binding domain of Myflu POU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU DBD

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Salhel-POU

Plasmid

#231541

Purpose

Expresses the DNA binding domain of Salhel POU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU DBD

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Salhel-I-Sox2HMG

Plasmid

#231533

Purpose

Expresses chimeric Sox2 HMG in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

mSox2 HMG flanked with N- and C-termini of Salhel-I

Use

Lentiviral

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-Salhel-Sox-I

Plasmid

#231515

Purpose

Expresses Salpingoeca helianthica full-length Sox-I in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I

Use

Lentiviral

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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