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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,843 results for: abr

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Showing: 1721 - 1760 of 1843 results

  5. pIgG1-2.1

    Plasmid
    #225831
    Purpose
    Mouse IgG1 chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG- and Strep2-tagged monoclonal antibodies for use as tag-specific ELISA standards.
    Depositor
    Gabriel Victora
    Article
    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:
    Insert
    Mouse anti-CGG, IgG1 heavy chain
    Expression
    Mammalian
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225831-plasmid-map-sequence-id-447625

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  11. MSP9-bio

    Plasmid
    #47740
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP9 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP9
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47740-plasmid-map-sequence-id-68875

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  15. GABBR1-Tango

    Plasmid
    #66287
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GABBR1 (GABBR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66287-plasmid-map-sequence-id-261739

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  24. GABBR1-DuET

    Plasmid
    #213247
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GABBR1 (GABBR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213247-plasmid-map-sequence-id-430899

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  27. pLV-TetOn-ND-Cdt1-mCherry

    Plasmid
    #193761
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193761-plasmid-map-sequence-id-380233

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  28. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

    Plasmid
    #74606
    Purpose
    expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Y101A, Y103A, W120A
    Promoter
    SFFV
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74606-plasmid-map-sequence-id-151888

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  33. pCAG_SRGAP2A_GAP(R527L_K566A)

    Plasmid
    #222617
    Purpose
    Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.
    Depositor
    Franck Polleux
    Article
    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1
    Insert
    SRGAP2 (SRGAP2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Point mutations R527L and K566A (Rac1-specific GA…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222617-plasmid-map-sequence-id-448408

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  37. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

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  38. pCW-ND-Cdt1-mCherry-Puro

    Plasmid
    #193766
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193766-plasmid-map-sequence-id-380221

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  39. pCW-ND-Cdt1-HA-Puro

    Plasmid
    #193767
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-HA (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193767-plasmid-map-sequence-id-380216

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Showing: 1721 - 1760 of 1843 results