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mEmerald-Desmocollin1-N-18
Plasmid#54062PurposeLocalization: Cadherin, Excitation: 487, Emission: 509DepositorInsertDesmocollin1 (DSC1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZMU-Dest
Plasmid#105089PurposeYeast expression vector for Gateway cloning and SUS bridge assaysDepositorTypeEmpty backboneUseTagsmycExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-LA-nanoLuc-RA
Plasmid#128692PurposeDonor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locusDepositorInsertapobb.1 NanoLuc
UseMultisite-gateway assemblyTagsNanoLucExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmin/pAS2-1
Plasmid#97407PurposeDesmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertDesmin (DES Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
Destination Vector
Plasmid#125754PurposeDestination vector for assembling gRNA fragments in the second round of assembly.DepositorInsertpTDH3-GFPdropout-tTDH1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL8A
Plasmid#67466PurposeMammalian expression of hArl8 with stop codon (native expression)DepositorInsertARL8A (ARL8A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-Arl13b17
Plasmid#40875DepositorInsertArl13b (Arl13b Mouse)
UseTagsGFPExpressionMammalianMutationcontains Arl13b amino acids 1-199PromoterCMVAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDest_pK7WG2_pRPS5A_CTP OleGFP
Plasmid#171723PurposeptpTALECD vector assembly, Destination vector;DepositorInsertRPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest490-OPN-NT
Plasmid#17594DepositorInsertOPN-NT (SPP1 Human)
UseTagsFlagExpressionMammalianMutationBase pairs 49-498PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFcpB-DEST
Plasmid#90098PurposeGateway destination vector featuring FcpB promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.DepositorTypeEmpty backboneUseDestination vector for gateway expressionTagsExpressionMutationPromoterFcpBAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Desmoglein2_tail-GST
Plasmid#36990DepositorInsertDsg2 (DSG2 Human)
UseTagsGSTExpressionBacterialMutationIntracellular domain of DSG2 (nucleotides 1911–35…PromoterAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARFRP1
Plasmid#67471PurposeMammalian expression of hArfrp with stop codon (native expression)DepositorInsertARFRP1 (ARFRP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AK570A
Plasmid#14104DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Lys570 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF4
Plasmid#67455PurposeMammalian expression of hArf4 with stop codon (native expression)DepositorInsertARF4 (ARF4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
Plasmid#65662PurposeFLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SAR1
Plasmid#67388PurposeExpression of native hSar1 in E. coliDepositorInsertSAR1 (SAR1A Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
GGDestX1-Amp
Plasmid#157652PurposeGolden Gate assembly and cell-free (bacterial) expression of fusion-proteinsDepositorTypeEmpty backboneUseCell-free expression (e. coli sigma-70)TagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_TLOC1 (220aa)
Plasmid#45273DepositorInsertTLOC1 (SEC62 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Desmocollin2_tail-GST
Plasmid#36992DepositorInsertDsc2 (DSC2 Human)
UseTagsGSTExpressionBacterialMutationIntracellular domain of Dsc2 (nt 2,603–3,158)PromoterAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDest490-OPN-CT
Plasmid#17593DepositorInsertOPN-CT (SPP1 Human)
UseTagsFlagExpressionMammalianMutationBase pairs 631–942PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS
Plasmid#59388PurposeDestination vector with RBPMS for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Desmocollin1-N-18
Plasmid#57370PurposeLocalization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertDesmocollin1 (DSC1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCWX-R4-DEST-R2-PG
Plasmid#45957DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST15 hSlp4-a
Plasmid#40046DepositorInserthSlp4-a (SYTL4 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Nedd4.2F823A
Plasmid#165104Purposebacterial expression of a GST fusion of Human Nedd4.2d F823ADepositorInsertNedd4.2d F823A (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationF823A; mutation for destabilization of trimer fo…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL1
Plasmid#67458PurposeMammalian expression of hArl1 with stop codon (native expression)DepositorInsertARL1 (ARL1 Human)
UseTagsExpressionMammalianMutationbp 231 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestRFP_HuB
Plasmid#65764Purposeexpresses RFP-tagged HuB in mammalian cellsDepositorInsertELAVL2 (ELAVL2 Human)
UseTagsExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-Arl13b20
Plasmid#40876DepositorInsertArl13b (Arl13b Mouse)
UseTagsGFPExpressionMammalianMutationdeleted Arl N-terminal domain; added stop codonPromoterCMVAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Desmocollin1-N-18
Plasmid#57608PurposeLocalization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertDesmocollin1 (DSC1 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBW700_pCAG-pDest-U1-UX
Plasmid#87578PurposepCAG destination vector (U1-UX)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSWt1-Dest
Plasmid#59764PurposePCR template for amplifying the attL1-em7-kanr-attL2-pA cassetteDepositorInsertattL1-em7-kanR-attL2-pA
UseTagsExpressionMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
SYNJ1-170_pCSdest
Plasmid#53822DepositorInsertSYNJ1-170 (SYNJ1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Munc18cF119E-pDEST27
Plasmid#85625PurposeGST tagged Munc18c with a F119E mutationDepositorInsertMunc18c (Stxbp3 Mouse)
UseTagsGSTExpressionMammalianMutationF119EPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Desmocollin1-C-18
Plasmid#55027PurposeLocalization: Cadherin, Excitation: 587, Emission: 610DepositorInsertDesmocollin1 (DSC1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF6
Plasmid#67457PurposeMammalian expression of hArf6 with stop codon (native expression)DepositorInsertARF6 (ARF6 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 5A
Plasmid#60651PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated five serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMYA5T2/pDEST15
Plasmid#98109PurposeExpress CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteriaDepositorInsertCMYA5 (CMYA5 Human)
UseTagsGSTExpressionBacterialMutationrs10043986 (NP_705838.3:p.Pro4063Leu)PromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos2-Desmocollin1-C-18
Plasmid#57369PurposeLocalization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertDesmocollin1 (DSC1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFRT-TODestGFP_RBPMS
Plasmid#65744Purposeexpresses GFP-tagged RBPMS in mammalian cellsDepositorInsertRBPMS (RBPMS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only
